促性腺激素释放激素II对子宫内膜异位症患者子宫内膜间质细胞的体外凋亡作用
In vitro apoptosis effects of GnRHII on endometrial stromal cells from patients with endometriosis.
作者信息
Huang Fengying, Zou Ying, Wang Huanping, Cao Jing, Yin Tuanfang
机构信息
Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China, 410011.
出版信息
Int J Clin Exp Pathol. 2013 Jul 15;6(8):1603-9. Print 2013.
PURPOSE
To study the effect of gonadotropin-releasing hormone II (GnRHII) on the cell apoptosis of ectopic, eutopic and normal endometrial stromal cells cultured in vitro from endometriosis patients, and to provide theoretical basis for exploring new treatments for endometriosis (EMs).
METHODS
Ectopic, eutopic and normal endometrial stromal cells were isolated, cultured and identified in vitro, then treated with different concentrations of GnRHII (0, 10(-10) M, 10(-8) M and 10(-6) M). Cell apoptosis was detected by Hoechst staining and flow cytometry.
RESULTS
GnRHII increased apoptosis in ectopic, eutopic and normal stromal cells in a dosage-dependent manner (P<0.05), and apoptosis of ectopic stroma cells was significantly higher than that of eutopic and normal cells (P<0.05); apoptosis in eutopic and normal cells had no different (P>0.05).
CONCLUSION
GnRHII can significantly induce apoptosis in ectopic, eutopic and normal endometrial stromal cells from patients with endometriosis, especially to the ectopic.
目的
研究促性腺激素释放激素II（GnRHII）对子宫内膜异位症患者体外培养的异位、在位及正常子宫内膜间质细胞凋亡的影响，为探索子宫内膜异位症（EMs）新的治疗方法提供理论依据。
方法
体外分离、培养并鉴定异位、在位及正常子宫内膜间质细胞，然后用不同浓度的GnRHII（0、10⁻¹⁰ M、10⁻⁸ M和10⁻⁶ M）进行处理。采用Hoechst染色和流式细胞术检测细胞凋亡。
结果
GnRHII以剂量依赖方式增加异位、在位及正常间质细胞的凋亡（P<0.05），异位间质细胞的凋亡明显高于在位及正常细胞（P<0.05）；在位和正常细胞的凋亡无差异（P>0.05）。
结论
GnRHII能显著诱导子宫内膜异位症患者异位、在位及正常子宫内膜间质细胞凋亡，尤其是对异位细胞。
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