Lanzendorf S E, Gliessman P M, Archibong A E, Alexander M, Wolf D P
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton, Oregon.
Mol Reprod Dev. 1990 Jan;25(1):61-6. doi: 10.1002/mrd.1080250111.
Electroejaculation is an accepted method of semen collection from nonhuman primates. Although both penile and rectal probe stimulation techniques have been used, there has been a general lack of consistency and detail regarding their application. This report describes the collection, processing, and evaluation of rhesus monkey semen contrasting two methods of penile electroejaculation: 1) a constant-voltage method where stimulus current is a variable and 2) a constant-current method where stimulus current is operator-controlled. The constant-current method was the more efficient procedure, requiring a lower stimulus current for successful electroejaculation. The influence on semen quality of potentially toxic agents used in the procedure, surgical glove powder and electrolyte cream, was tested; both were detrimental as measured by motility loss. No correlation was found between coagula volume and sperm numbers. The intra- and interanimal variability in semen samples from six monkeys was also evaluated. Penile electroejaculation, combined with control of stimulus current, provides a consistent, successful, and humane method for the collection of semen in the rhesus monkey.
电射精是一种公认的从非人灵长类动物采集精液的方法。尽管阴茎刺激和直肠探头刺激技术都已被使用，但在它们的应用方面普遍缺乏一致性和详细描述。本报告描述了恒河猴精液的采集、处理和评估，对比了两种阴茎电射精方法：1）恒压法，其中刺激电流是可变的；2）恒流法，其中刺激电流由操作者控制。恒流法是更有效的程序，成功进行电射精所需的刺激电流更低。测试了该程序中使用的潜在有毒物质手术手套粉末和电解质乳膏对精液质量的影响；通过活力丧失测量，两者都有损害。未发现凝块体积与精子数量之间存在相关性。还评估了六只猴子精液样本的动物内和动物间变异性。阴茎电射精结合刺激电流控制，为恒河猴精液采集提供了一种一致、成功且人道的方法。
