基于 MS 的合成报告肽的蛋白水解降解监测，用于血浆和血清标本的质量控制。
MS-based monitoring of proteolytic decay of synthetic reporter peptides for quality control of plasma and serum specimens.
机构信息
Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1, 68167 Mannheim, Germany.
出版信息
Am J Clin Pathol. 2013 Sep;140(3):314-23. doi: 10.1309/AJCPOS9Z5KVZSFSC.
OBJECTIVES
To determine the preanalytical quality of serum and plasma by monitoring the time-dependent ex vivo decay of a synthetic reporter peptide (RP) with liquid chromatography/mass spectrometry (LC/MS).
METHODS
Serum and plasma specimens were spiked with the RP and proteolytic fragments were monitored with LC/MS at different preanalytical time points ranging from 2 to 24 hours after blood withdrawal.
RESULTS
The concentration of fragments changed in a time-dependent manner, and respective peptide profiles were used to classify specimens according to their preanalytical time span. Classification accuracy was high, with values always above 0.89 for areas under receiver operating characteristic curves.
CONCLUSIONS
This "proteomics degradation clock" can be used to estimate the preanalytical quality of serum and plasma and might have impact on quality control procedures of biobanking repositories.
目的
通过监测合成报告肽（RP）在体外随时间的衰减情况，来确定血清和血浆的分析前质量。
方法
将 RP 和蛋白水解片段加入血清和血浆标本中，在采血后 2 至 24 小时的不同分析前时间点，用液相色谱/质谱（LC/MS）进行监测。
结果
片段的浓度随时间呈依赖性变化，并且各自的肽谱可用于根据分析前时间跨度对标本进行分类。分类准确性很高，受试者工作特征曲线下的面积值始终高于 0.89。
结论
这种“蛋白质组学降解钟”可用于估计血清和血浆的分析前质量，并可能对生物库存储库的质量控制程序产生影响。
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