Cheng Chi-Fang, Wu Kai-Ming, Chen Yen-Ting, Hung Shan-Ling
Department of Community Dentistry, Zhong-Xiao Branch, Taipei City Hospital, Taipei, Taiwan; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.
School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan; Taipei Municipal Hospital, Taipei, Taiwan; Keelung City Municipal Hospital, Keelung City, Taiwan.
J Formos Med Assoc. 2015 Jan;114(1):35-45. doi: 10.1016/j.jfma.2013.07.010. Epub 2013 Aug 19.
BACKGROUND/PURPOSE: Bacterial contamination of sites undergoing guided tissue regeneration (GTR) therapy may reduce the efficiency of periodontal regeneration. This study compared bacterial adhesion onto various GTR membranes incorporated with antibiotics.
Three barrier membranes, including expanded polytetrafluoroethylene (ePTFE) membrane, collagen membrane, and glycolide fiber membrane, were loaded with tetracycline or amoxicillin. The adhesion of Streptococcus mutans and Aggregatibacter actinomycetemcomitans onto the GTR membranes with or without antibiotics was analyzed using the scanning electron microscopy (SEM) analysis.
The SEM analysis showed no apparent alteration in the physical structure of the membranes loaded with antibiotics. Both S. mutans and A. actinomycetemcomitans attached best on the collagen membranes, followed by the ePTFE membranes, and then the glycolide fiber membranes without antibiotics. Moreover, higher numbers of bacteria were observed on the fibril areas than on the laminar areas of the ePTFE membranes. The amounts of attached bacteria on the GTR membranes increased after longer incubation. Incorporation of tetracycline or amoxicillin greatly reduced the adhesion of S. mutans and A. actinomycetemcomitans onto all of the GTR membranes examined.
Incorporation of tetracycline or amoxicillin greatly reduced adhesion of S. mutans or A. actinomycetemcomitans on the ePTFE, glycolide fiber, or collagen membranes. This finding indicates that it is valuable and effective to use the antibiotic-loaded GTR membranes for periodontal regeneration therapy.
背景/目的：引导组织再生（GTR）治疗部位的细菌污染可能会降低牙周再生的效率。本研究比较了细菌在掺入抗生素的各种GTR膜上的黏附情况。
三种屏障膜，包括膨体聚四氟乙烯（ePTFE）膜、胶原膜和乙交酯纤维膜，分别加载四环素或阿莫西林。使用扫描电子显微镜（SEM）分析变形链球菌和伴放线聚集杆菌在有无抗生素的GTR膜上的黏附情况。
SEM分析显示，加载抗生素的膜的物理结构没有明显改变。变形链球菌和伴放线聚集杆菌在胶原膜上的黏附最好，其次是ePTFE膜，然后是未添加抗生素的乙交酯纤维膜。此外，在ePTFE膜的原纤维区域观察到的细菌数量多于层状区域。延长孵育时间后，GTR膜上附着的细菌数量增加。四环素或阿莫西林的掺入大大降低了变形链球菌和伴放线聚集杆菌在所有检测的GTR膜上的黏附。
四环素或阿莫西林的掺入大大降低了变形链球菌或伴放线聚集杆菌在ePTFE、乙交酯纤维或胶原膜上的黏附。这一发现表明，使用加载抗生素的GTR膜进行牙周再生治疗是有价值且有效的。
