Choi Saehae, Song Jinsue, Son Se-Young, Park Il Yeong, Yamashita Eiki, Lee Soo Jae
College of Pharmacy, Chungbuk National University, 410 Seungbong, Heungduk, Cheongju 361-763, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):1049-51. doi: 10.1107/S1744309113023038. Epub 2013 Aug 23.
Snail is a C2H2-type zinc finger transcriptional repressor that induces epithelial-mesenchymal transition by repression of E-cadherin expression levels during embryonic development and tumour progression. Snail is imported into the nucleus by importin β through direct binding with its four zinc finger domain. The complex between importin β and Snail four zinc finger domain was crystallized in order to understand the nuclear transport mechanism of Snail. The constituents of the complex were separately expressed and were then co-purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group C2, with unit-cell parameters a = 228.2, b = 77.5, c = 72.0 Å, β = 100.9° and diffracted to 2.5 Å resolution.
Snail是一种C2H2型锌指转录抑制因子，在胚胎发育和肿瘤进展过程中，它通过抑制E-钙黏蛋白的表达水平来诱导上皮-间质转化。Snail通过其四个锌指结构域与importin β直接结合，从而被importin β转运到细胞核中。为了了解Snail的核转运机制，对importin β与Snail四个锌指结构域之间的复合物进行了结晶。复合物的组成成分分别表达，然后通过悬滴气相扩散法进行共纯化和结晶。晶体属于空间群C2，晶胞参数为a = 228.2、b = 77.5、c = 72.0 Å，β = 100.9°，衍射分辨率为2.5 Å。
