[奥沙利铂对肝癌细胞系HepG2细胞周期的影响]
[Effect of Oxaliplatin on cell cycle of hepatocellular carcinoma cell line HepG2].
作者信息
Gao Jie, Wang Rui, Yang Qingling, Chen Changjie, Wu Qiong
机构信息
Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2013 Jul;42(4):437-42. doi: 10.3785/j.issn.1008-9292.2013.04.011.
OBJECTIVE
To investigate the effect of Oxaliplatin (L-OHP) on cell cycle in hepatocellular carcinoma cell line HepG2 and the involved mechanism.
METHODS
Inhibitory effect of L-OHP on the proliferation of HepG2 cells was determined by MTT assay. Cell cycle distribution was shown by flow FCM. The expression levels of cyclinD1, CDK2, CDK4, p16, p21, p53 were detected by RT-PCR and Western blot.
RESULTS
MTT method revealed that L-OHP inhibited proliferation of hepatocellular carcinoma HepG2 cells in a dose- and time-dependent manner. L-OHP induced S cell cycle arrest in HepG2 cell; down-regulated the levels of CDK4, cyclinD1 and up-regulated the levels of p21, p53. There were no significant changes of CDK2 and p16 after L-OHP treatment.
CONCLUSION
L-OHP inhibits the proliferation of HepG2 cells by blocking cell at S stage, which may be resulted from the activity of CDK4, CyclinD1 and p21.
目的
探讨奥沙利铂（L-OHP）对肝癌细胞系HepG2细胞周期的影响及其作用机制。
方法
采用MTT法检测L-OHP对HepG2细胞增殖的抑制作用。通过流式细胞术（FCM）分析细胞周期分布。采用RT-PCR和Western blot检测细胞周期蛋白D1（cyclinD1）、细胞周期蛋白依赖性激酶2（CDK2）、细胞周期蛋白依赖性激酶4（CDK4）、p16、p21、p53的表达水平。
结果
MTT法显示L-OHP以剂量和时间依赖性方式抑制肝癌HepG2细胞的增殖。L-OHP诱导HepG2细胞S期细胞周期阻滞；下调CDK4、cyclinD1水平，上调p21、p53水平。L-OHP处理后CDK2和p16无明显变化。
结论
L-OHP通过将细胞阻滞于S期抑制HepG2细胞增殖，这可能与CDK4、细胞周期蛋白D1及p21的活性有关。
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