从质粒或聚合酶链反应扩增的DNA进行体外转录。
In vitro transcription from plasmid or PCR-amplified DNA.
作者信息
Brunelle Julie L, Green Rachel
机构信息
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
出版信息
Methods Enzymol. 2013;530:101-14. doi: 10.1016/B978-0-12-420037-1.00005-1.
Abstract
This protocol describes the synthesis and purification of RNAs using plasmid DNA or PCR-amplified DNA as a template. This procedure should give NTP-free, full-length RNA for all sizes of RNA. This protocol is derived from Milligan and Uhlenbeck, the classic paper on T7 transcription reactions, with modifications.
摘要
本方案描述了以质粒DNA或PCR扩增的DNA为模板合成和纯化RNA的方法。该程序应为所有大小的RNA提供无NTP的全长RNA。本方案源自Milligan和Uhlenbeck关于T7转录反应的经典论文，并做了修改。
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