盐酸安非他酮中间氯苯甲酸的液相色谱测定法。

Liquid chromatographic determinations of meta-chlorobenzoic acid in bupropion hydrochloride.

作者信息

Sinha Sukumar, Parashar Vikas, Reddy K Swamy, Khan Mohammad A, Haque S K Manirul

机构信息

Department of Analytical Research Lab, Glenmark Generics Ltd, Glenmark Research Centre, Plot A - 607, TTC Industrial Area, MIDC, Mahape, Navi Mumbai, Maharashtra, India.

出版信息

J Nat Sci Biol Med. 2013 Jul;4(2):435-9. doi: 10.4103/0976-9668.117018.

DOI:10.4103/0976-9668.117018

PMID:24082747

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3783795/

Abstract

A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride.

摘要

描述了一种测定盐酸安非他酮中间氯苯甲酸的简单、灵敏的方法。使用由正己烷和乙醇（1000:50，v/v）组成的流动相，流速为1.0 ml/min，在Chiralpak ADH（250×4.6 mm）柱上实现间氯苯甲酸的色谱分离。在235 nm处监测吸光度。该方法在中间氯苯甲酸的定量限浓度范围（5.0 μg/ml至15.0 μg/ml）内呈线性，相关系数大于0.99。该方法在测定盐酸安非他酮中的间氯苯甲酸时比美国药典方法更具选择性和准确性。