No detection of the NS5B S282T mutation in treatment-naïve genotype 1 HCV/HIV-1 coinfected patients using deep sequencing.

BACKGROUND

The S282T mutation is the main variant described associated with resistance to nucleos(t)ide analogues hepatitis C virus (HCV) NS5B polymerase inhibitors.

OBJECTIVE

We aimed here to investigate whether this substitution pre-existed in treatment naive HCV/HIV-1 coinfected patients.

STUDY DESIGN

NS5B polymerase deep sequencing was performed at a median coverage per base of 4471 in 16 patient samples.

RESULTS

No S282T variant was detected in the 16 analyzed samples.

CONCLUSION

This finding is in agreement with the high genetic barrier of nucleoside analogues NS5B polymerase inhibitors and the clinical efficacy of these compounds.