Robertson B, Arvidsson J
Brain Res. 1985 Nov 25;348(1):44-51. doi: 10.1016/0006-8993(85)90357-9.
Horseradish peroxidase conjugates of either the lectin wheat germ agglutinin (WGA-HRP) or choleragenoid (B-HRP) have been shown to be sensitive neuroanatomical tracers. In the present study a comparison was made between these two conjugates as transganglionic tracers in trigeminal primary sensory neurons following injection into the rat mystacial vibrissae skin. Differences between the two tracers were observed in the labeling of cell bodies in the trigeminal ganglion. Injection of WGA-HRP resulted in labeling of predominantly small cell bodies, whereas B-HRP gave rise to labeling of somewhat larger cell bodies. By increasing the concentration of the injected WGA-HRP solution the number of labeled cells increased substantially, while a corresponding increase in the concentration of B-HRP resulted in a relatively small increase in the number of labeled cells. WGA-HRP injection resulted in labeling of primary afferents mainly in the substantia gelatinosa of the trigeminal subnucleus caudalis. When the concentration of the injected WGA-HRP solution was increased, labeling was also observed in the marginal and magnocellular zones. Following B-HRP injection, labeling was only observed in the magnocellular zone and innermost part of the substantia gelatinosa. This general pattern of labeling was the same when the concentration of the B-HRP solution was increased.
凝集素小麦胚凝集素（WGA-HRP）或类霍乱毒素（B-HRP）的辣根过氧化物酶偶联物已被证明是敏感的神经解剖学示踪剂。在本研究中，将这两种偶联物作为经神经节示踪剂注入大鼠触须皮肤后，对三叉神经初级感觉神经元进行了比较。在三叉神经节细胞体的标记中观察到了两种示踪剂之间的差异。注射WGA-HRP主要导致小细胞体的标记，而B-HRP则导致稍大细胞体的标记。通过增加注射的WGA-HRP溶液的浓度，标记细胞的数量大幅增加，而B-HRP浓度的相应增加导致标记细胞数量的相对较小增加。WGA-HRP注射主要导致三叉神经尾侧亚核胶状质中初级传入纤维的标记。当注射的WGA-HRP溶液浓度增加时，在边缘区和大细胞区也观察到标记。注射B-HRP后，仅在大细胞区和胶状质的最内部观察到标记。当B-HRP溶液浓度增加时，这种总体标记模式是相同的。
