咖啡属叶绿体 DNA 的遗传和限制片段长度多态性

Inheritance and restriction fragment length polymorphism of chloroplast DNA in the genus Coffea L.

机构信息

ORSTOM, Laboratoire de ressources génétiques et d'amélioration des plantes tropicales, BP 5045, F-34032, Montpellier, France.

出版信息

Theor Appl Genet. 1996 Sep;93(4):626-32. doi: 10.1007/BF00417958.

DOI:10.1007/BF00417958

PMID:24162358

Abstract

CpDNA variation among 52 tree samples belonging to 25 different taxa of Coffea and two species of Psilanthus was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region. Twelve variable characters were distinguished allowing the identification of 12 different plastomes. The low sequence divergence observed might suggest that Coffea is a young genus. The results were in contradiction with the present classification into two genera. Additionally, cpDNA inheritance was studied in interspecific hybrids between C. arabica and C. canephora, and in an intraspecific progeny of C. canephora, using PCR-based markers. Both studies showed exclusively maternal inheritance of cpDNA.

摘要

通过 RFLP 分析，对 52 个树样本（属于 25 个不同的咖啡属和 2 个 Psilanthus 种）的整个叶绿体基因组和 atpB-rbcL 基因间隔区进行了 cpDNA 变异分析。区分了 12 个可变特征，确定了 12 个不同的质体基因组。观察到的低序列分歧可能表明咖啡属是一个年轻的属。这些结果与目前分为两个属的分类法相矛盾。此外，还通过基于 PCR 的标记研究了 C. arabica 和 C. canephora 种间杂种以及 C. canephora 种内后代中的 cpDNA 遗传。这两项研究均显示 cpDNA 仅为母系遗传。