Department of Plant Science, McGill University, Macdonald Campus, 21 111 Lakeshore, H9X 3V9, Ste-Anne-de-Bellevue, QC.
Theor Appl Genet. 1993 Feb;85(8):976-84. doi: 10.1007/BF00215037.
We investigated random amplified polymorphic DNA (RAPD) in 27 inbred barley lines with varying amounts of common ancestry and in 20 doubled-haploid (DH) lines from a biparental cross. Of 33 arbitrary 10 base primers that were tested, 19 distinguished a total of 31 polymorphisms. All polymorphisms were scored as dominant genetic markers except for 1, where Southern analysis indicated the presence of two codominant amplification products. The inheritance of 19 RAPD polymorphisms and one morphological trait was studied in the DH lines. There was no evidence for segregation distortion, but a group of four tightly linked loci was detected. The frequencies of RAPD polymorphism in pairs of inbred lines were used to compute values of genetic distance (d), which were compared to kinship coefficients (r) between the same pairs of lines. A linear relationship between r and d was evident, but low values of r gave poor predictions of d. Cluster analysis showed that groups of inbred lines based on r were similar to those based on d with some notable exceptions. RAPD markers can be used to gain information about genetic similarities or differences that are not evident from pedigree information.
我们研究了 27 个具有不同共同祖先数量的近交大麦品系和 20 个来自双交的加倍单倍体 (DH) 系的随机扩增多态性 DNA (RAPD)。在测试的 33 个任意 10 碱基引物中，有 19 个总共区分了 31 个多态性。所有多态性都被标记为显性遗传标记，除了 1 个，其中 Southern 分析表明存在两个共显性扩增产物。在 DH 系中研究了 19 个 RAPD 多态性和 1 个形态特征的遗传。没有分离失真的证据，但检测到一组四个紧密连锁的基因座。在一对近交系中 RAPD 多态性的频率用于计算遗传距离 (d) 值，并将其与同一对系之间的亲缘系数 (r) 进行比较。r 和 d 之间存在明显的线性关系，但 r 的低值对 d 的预测效果不佳。聚类分析表明，基于 r 的近交系组与基于 d 的组相似，但也有一些明显的例外。RAPD 标记可用于获取遗传相似性或差异的信息，这些信息从系谱信息中并不明显。
