利用同步辐射对小鼠进行体内冠状动脉血管造影。
Mouse coronary angiography in vivo using synchrotron radiation.
作者信息
Xu Lijun, Zhang Andi, Du Guohao, Xie Honglan, Chen Ying
机构信息
Department of Biomedical Engineering, School of Medicine, Shanghai Jiao Tong University, No.280, South Chongqing RD, Shanghai, China.
出版信息
Biomed Mater Eng. 2014;24(1):1341-9. doi: 10.3233/BME-130937.
PURPOSE
To establish a method for mouse coronary angiography in vivo using synchrotron radiation, which is essential for physiological and pathological research on coronary diseases.
METHODS
- The imaging parameters (e.g., photon energy, spatial resolution of the detector, and injection rate of contrast agent) optimal for the quality of acquired images in a simulation were determined. 2) Through animal experiments, the effectiveness of these optimal parameters and the repeatability of in vivo coronary angiography were verified. 3) An algorithm for background subtraction and contrast enhancement was designed and employed to compensate for the effects of interference and the effective information extracted used for diagnosing coronary disease.
RESULTS AND CONCLUSIONS
An optimal set of the imaging parameters was finally determined: photon energy of 33-34 keV, detector's spatial resolution of 30 μm or higher, image capture rate of 20 f/s or more, concentration of lopamidol solution of 75% as contrast agent and a pulse injection of contrast agent at a high rate.
目的
建立一种利用同步辐射进行小鼠体内冠状动脉血管造影的方法，这对于冠心病的生理和病理研究至关重要。
方法
1）在模拟中确定获取图像质量最佳的成像参数（如光子能量、探测器空间分辨率和造影剂注射速率）。2）通过动物实验，验证这些最佳参数的有效性以及体内冠状动脉血管造影的可重复性。3）设计并采用一种背景减除和对比度增强算法，以补偿干扰影响并提取用于诊断冠心病的有效信息。
结果与结论
最终确定了一组最佳成像参数：光子能量为33 - 34 keV，探测器空间分辨率为30μm或更高，图像采集速率为20帧/秒或更高，作为造影剂的洛美多醇溶液浓度为75%，并采用高速脉冲注射造影剂。
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