[人参皂苷Rg1通过基因芯片促进人神经干细胞的分子靶点研究]
[Study on molecular target promoting human neural stem cells of ginsenoside Rg1 by gene chip].
作者信息
Li Ying-Bo, Zhao Xiang-Qin, Jiang Ying-Hong, Chen Di, Wang Sha-Li
机构信息
Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Aug;38(16):2701-5.
OBJECTIVE
To screen out main molecular target promoting human neural stem cells (NSCs) of ginsenoside Rg1 by using the gene chip technology.
METHOD
First, MTT assay was adopted to screen out the optimal concentration of Rg1-promoted NSC proliferation (120 mg x L(-1)). Then, on the 7th day after the Rg1-promoted NSC proliferation, the expression of target genes was observed by the gene chip technology. The most important target gene and signal transduction pathways were screened out through the data calculations.
RESULT
On the 7th day after the Rg1-promoted NSC proliferation, obtained 440 differential genes, 266 significantly upregulated genes and 174 significantly down-regulated genes. HES1 gene, CAMP (cyclic adenosine monophosphate)-PKA (protein kinase A) and PI3K (phosphatidylinositol 3 kinase)-AKT signal transduction pathways were closely related to the NSC proliferation.
CONCLUSION
The differentially expressed genes screened out by gene chip may provide new clues for studies on molecular mechanism of ginsenoside Rg1-promoted NSCs proliferation.
目的
运用基因芯片技术筛选出促进人参皂苷Rg1作用于人神经干细胞（NSCs）增殖的主要分子靶点。
方法
首先，采用MTT法筛选出Rg1促进NSC增殖的最佳浓度（120 mg·L⁻¹）。然后，在Rg1促进NSC增殖后的第7天，运用基因芯片技术观察靶基因的表达情况。通过数据计算筛选出最重要的靶基因和信号转导通路。
结果
在Rg1促进NSC增殖后的第7天，获得440个差异基因，其中266个基因显著上调，174个基因显著下调。HES1基因、CAMP（环磷酸腺苷）-PKA（蛋白激酶A）和PI3K（磷脂酰肌醇3激酶）-AKT信号转导通路与NSC增殖密切相关。
结论
基因芯片筛选出的差异表达基因可能为人参皂苷Rg1促进NSCs增殖的分子机制研究提供新线索。
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