State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, China,
Environ Sci Pollut Res Int. 2014 Mar;21(5):3484-90. doi: 10.1007/s11356-013-2344-z. Epub 2013 Nov 19.
Lead (Pb) and decabromodiphenyl ether (BDE209) are the main contaminants at e-waste recycling sites, and their potential toxicological effects on terrestrial organisms have received extensive attention. However, the impact on earthworms of exposure to the two chemicals remains almost unknown. Therefore, indoor incubation tests were performed on control and contaminated soil samples to determine the Pb accumulations and toxic effects by earthworms in the presence of BDE209 for the first time. The results have demonstrated that BDE209 presence can affect Pb bioaccumulation efficiency compared with exposure to Pb alone. The Pb contents in earthworms had a highly positive correlation with the Pb concentrations in soils. For different Pb doses, almost contrary response trends were found for Pb uptake examined separately on day 7 or 28, and dose-effect relationships were clearly observed in the presence of BDE209. After 7 days of exposure, the earthworm bodies receiving 1-mg kg(-1) BDE209 dose showed significantly lower Pb contents (average = 175.85 mg kg(-1)) and bioaccumulation factor (average = 0.574) than those receiving non-BDE209 treatments (217.39 mg kg(-1) and 1.209, respectively). As the incubation time extended, the influence of BDE209 presence on Pb uptake gradually declined. Additionally, either single or combined exposure to both chemicals can affect the protein synthesis in earthworms (p < 0.01), while different levels of BDE209 addition barely caused visible differences. The results of these observations have provided a basic understanding on the potential toxicological effects of joint Pb and BDE209 exposure on terrestrial invertebrates.
铅（Pb）和十溴二苯醚（BDE209）是电子废物回收场的主要污染物，它们对陆地生物的潜在毒理学影响受到了广泛关注。然而，这两种化学物质对蚯蚓的影响几乎未知。因此，首次进行了室内孵化试验，以确定在 BDE209 存在的情况下，两种化学物质对蚯蚓的 Pb 积累和毒性影响。结果表明，与单独暴露于 Pb 相比，BDE209 的存在会影响 Pb 的生物积累效率。蚯蚓体内的 Pb 含量与土壤中的 Pb 浓度呈高度正相关。对于不同的 Pb 剂量，在第 7 天或第 28 天单独检测 Pb 吸收时，发现了几乎相反的响应趋势，并且在存在 BDE209 的情况下，明显观察到剂量-效应关系。暴露 7 天后，接受 1mg kg(-1) BDE209 剂量的蚯蚓体的 Pb 含量（平均值=175.85mg kg(-1))和生物积累系数（平均值=0.574)明显低于未接受 BDE209 处理的蚯蚓体（分别为 217.39mg kg(-1)和 1.209)。随着孵化时间的延长，BDE209 存在对 Pb 吸收的影响逐渐减弱。此外，无论是单一或联合暴露于这两种化学物质，都会影响蚯蚓的蛋白质合成（p<0.01），而添加不同水平的 BDE209 几乎不会导致明显差异。这些观察结果为了解 Pb 和 BDE209 联合暴露对陆地无脊椎动物的潜在毒理学影响提供了基础。
