Plant Biotechnology Section, Bio-Organic Division, Bhabha Atomic Research Centre, 400 085, Trombay, Bombay, India.
Plant Cell Rep. 1985 Apr;4(2):78-80. doi: 10.1007/BF00269211.
Stem segments, axillary buds and leaves excised from established shoot cultures of Morus indica were soaked in MS liquid medium containing benzyladenine (0.5, 1, 2 mg/1) and were cultured subsequently on semi solid medium of the same composition. Numerous shoot buds differentiated from leaf and axillary buds but stem segments were unresponsive. The shoot buds on isolation and culture developed into plantlets. Callus tissues which developed at the base of the leaf explant upon subculture also differentiated numerous shoot buds.
从已建立的桑树 shoot 培养物中切下的茎段、腋芽和叶片,浸泡在含有苄基腺嘌呤(0.5、1、2mg/1)的 MS 液体培养基中,然后随后在相同组成的半固体培养基上培养。叶片和腋芽中分化出许多芽,但茎段无反应。离体和培养的芽在分离和培养过程中发育成小植株。继代培养时,叶片外植体基部形成的愈伤组织也分化出许多芽。