维吾尔族儿童白血病患者白血病干细胞免疫表型及染色体核型特征

[Immunophenotyping of leukemic stem cells and chromosome karyotype characteristics in Uyghur leukemia pediatric patients].

作者信息

Hailiqiguli Nuriding, Yan Mei

机构信息

First Department of Pediatric Medcine, the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2013 Jul;35(7):501-4.

PMID:24257300

Abstract

OBJECTIVE

To study the immunophenotype and chromosome karyotype characteristics of leukemic stem cells (LSC) in Uyghur leukemia pediatric patients.

METHODS

The morphological features of LSC in culture in vitro was observed by flow cytometry. The immunophenotype was assessed by detective flow cytometry. The chromosome karyotype was analyzed by R-banding technique.

RESULTS

The LSC showed suspended floating colonies growing in the culture medium, and grew well and proliferated constantly in culture over 8 months. Among the 13 children with AML, there were 10 CD34(+)CD38(-)CD123(+) and CD33(+) cases, 10 CD44(+) cases, 10 CD96(+) cases, and 5 CD90(+) cases. Among the 13 children with B-ALL, there were 6 CD34(+)CD20(-)CD19(+) cases, 7 CD9(+) cases, and 5 CD123(+) cases. Among the 9 children with acute T lymphoblastic leukemia (T-ALL), there were 5 CD34(+)CD7(-) and CD90(+) cases, and 4 CD123(+) cases. Among the 13 cases of AML, 5 cases showed chromosome translocation t(15;17), one case chromosome translocation t(8;21), and 7 cases showed no chromosome karyotype abnormality. Among the 22 ALL cases, there were chromosome translocation t(12;21) in 1 case, t(9;22) in 3 case, hyperdiploid in 2 cases, and 16 cases without karyotype abnormalities. Twenty-nine children received induction remission therapy. Among them, 12 died, including 9 CD96(-)positive cases and 3 CD96(-)negative cases, with a statistically significant difference (P < 0.05).

CONCLUSIONS

The LSC of Uyghur leukemia pediatric patients in Xinjiang express CD9 and CD19 in ALL, and express CD123 and CD90 simultaneously in ALL and AML. The expression of CD96 is one of factors of poor prognosis.

摘要

目的

研究新疆维吾尔族儿童白血病患者白血病干细胞（LSC）的免疫表型及染色体核型特征。

方法

采用流式细胞术观察体外培养的LSC形态学特征；采用流式细胞仪检测免疫表型；采用R显带技术分析染色体核型。

结果

LSC在培养基中呈悬浮生长的集落，培养8个月以上生长良好且持续增殖。13例急性髓系白血病（AML）患儿中，10例为CD34(+)CD38(-)CD123(+)且CD33(+)，10例为CD44(+)，10例为CD96(+)，5例为CD90(+)。13例B淋巴细胞白血病（B-ALL）患儿中，6例为CD34(+)CD20(-)CD19(+)，7例为CD9(+)，5例为CD123(+)。9例急性T淋巴细胞白血病（T-ALL）患儿中，5例为CD34(+)CD7(-)且CD90(+)，4例为CD123(+)。13例AML患儿中，5例显示染色体易位t(15;17)，1例显示染色体易位t(8;21)，7例未显示染色体核型异常。22例ALL患儿中，1例显示染色体易位t(12;21)，3例显示t(9;22)，2例为超二倍体，16例无核型异常。29例患儿接受诱导缓解治疗，其中12例死亡，包括9例CD96(-)阳性病例和3例CD96(-)阴性病例，差异有统计学意义（P<0.05）。