Tang Xinyi, Tian Xinyu, Zhang Yue, Wu Wei, Tian Jie, Rui Ke, Tong Jia, Lu Liwei, Xu Huaxi, Wang Shengjun
Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University School of Medical Science and Laboratory Medicine, Zhenjiang 212013, China.
Clin Dev Immunol. 2013;2013:345347. doi: 10.1155/2013/345347. Epub 2013 Oct 30.
It was reported that IL-17 had been detected in the inflammatory infiltrates of patients with DM (dermatomyositis). In this study, we investigated the frequency of Th17 cells and the expression of microRNA-206 (miR-206) in DM patients. Firstly, we observed that the frequency of Th17 cells and the expression of transcription factors were increased significantly in the PBMCs of DM patients. Secondly, we found that there was a positive correlation between the percentages of Th17 cells and serum level of CK in DM patients. And the serum concentrations of IL-6, IL-1β, TGF-β, and IL-23, the important cytokines of Th17 differentiation, were increased in DM patients. It was predicted that Krüppel-like factor 4 (KLF4) is one of the multiple targets of miR-206. We detected the expression of miR-206 in DM patients, and it was decreased in the serum and PBMCs of DM patients. The augmented expression of KLF is accompanied by the attenuated expression of miR-206. Furthermore, a negative correlation between the percentages of Th17 cells and the expression of miR-206 in DM patients has been found. Taken together, these findings suggest the attenuated expression of miR-206, and the augmented frequency of Th17 cells in DM patients.
据报道，在皮肌炎（DM）患者的炎症浸润中已检测到白细胞介素-17（IL-17）。在本研究中，我们调查了DM患者中辅助性T细胞17（Th17）细胞的频率以及微小RNA-206（miR-206）的表达。首先，我们观察到DM患者外周血单个核细胞（PBMC）中Th17细胞的频率和转录因子的表达显著增加。其次，我们发现DM患者中Th17细胞百分比与血清肌酸激酶（CK）水平之间存在正相关。并且，DM患者中Th17分化的重要细胞因子白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、转化生长因子-β（TGF-β）和白细胞介素-23（IL-23）的血清浓度升高。据预测，Krüppel样因子4（KLF4）是miR-206的多个靶标之一。我们检测了DM患者中miR-206的表达，其在DM患者的血清和PBMC中降低。KLF的表达增加伴随着miR-206的表达减弱。此外，还发现DM患者中Th17细胞百分比与miR-206的表达之间存在负相关。综上所述，这些发现表明DM患者中miR-206表达减弱，Th17细胞频率增加。
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