Department of Chemistry, Faculty of Science, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia.
Int J Nanomedicine. 2013;8:4467-79. doi: 10.2147/IJN.S50837. Epub 2013 Nov 21.
Copper nanoparticle synthesis has been gaining attention due to its availability. However, factors such as agglomeration and rapid oxidation have made it a difficult research area. In the present work, pure copper nanoparticles were prepared in the presence of a chitosan stabilizer through chemical means. The purity of the nanoparticles was authenticated using different characterization techniques, including ultraviolet visible spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The antibacterial as well as antifungal activity of the nanoparticles were investigated using several microorganisms of interest, including methicillin-resistant Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella choleraesuis, and Candida albicans. The effect of a chitosan medium on growth of the microorganism was studied, and this was found to influence growth rate. The size of the copper nanoparticles obtained was in the range of 2-350 nm, depending on the concentration of the chitosan stabilizer.
由于其可用性,铜纳米粒子的合成受到了关注。然而,团聚和快速氧化等因素使其成为一个具有挑战性的研究领域。在本工作中,通过化学方法在壳聚糖稳定剂的存在下制备了纯铜纳米粒子。使用不同的表征技术,包括紫外可见光谱、透射电子显微镜、X 射线衍射、傅里叶变换红外光谱和场发射扫描电子显微镜,对纳米粒子的纯度进行了验证。使用几种感兴趣的微生物,包括耐甲氧西林金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌、肠炎沙门氏菌和白色念珠菌,研究了纳米粒子的抗菌和抗真菌活性。研究了壳聚糖介质对微生物生长的影响,发现壳聚糖介质会影响生长速度。所获得的铜纳米粒子的尺寸范围为 2-350nm,这取决于壳聚糖稳定剂的浓度。