槐耳多糖通过抑制 EMT 和 AEG-1 通路抑制肝癌 MHCC97-H 细胞转移。
Huaier polysaccharides suppresses hepatocarcinoma MHCC97-H cell metastasis via inactivation of EMT and AEG-1 pathway.
机构信息
Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, Beijing 100069, China.
出版信息
Int J Biol Macromol. 2014 Mar;64:106-10. doi: 10.1016/j.ijbiomac.2013.11.034. Epub 2013 Dec 7.
We have recently reported that astrocyte elevated gene-1 (AEG-1) might be an epithelial-mesenchymal transition (EMT) associated biomarker in human hepatocellular carcinoma (HCC), and play an important role in the progression of hepatocellular carcinoma. To extend our study, we examined here the anti-invasive and metastatic effects of Huaier polysaccharide (HP) on human HCC cell line MHCC97-H and explored its possible mechanism of action. Treatment with HP dose-dependently inhibited the proliferation, adhesion, migration and invasion of MHCC97-H cells in vitro. This was achieved not only by reducing the expression of AEG-1 and N-cadherin, but also by enhancing E-cadherin expression. Therefore, these data suggested that HP can inhibit the growth and metastatic potential of MHCC97-H cells through modulation of the AEG-1/EMT pathway.
我们最近报道称，星形细胞增强基因-1（AEG-1）可能是人类肝细胞癌（HCC）中上皮-间充质转化（EMT）相关的生物标志物，并在肝癌的进展中发挥重要作用。为了扩展我们的研究，我们在这里检查了槐耳多糖（HP）对人肝癌细胞系 MHCC97-H 的抗侵袭和转移作用，并探讨了其可能的作用机制。HP 处理呈剂量依赖性地抑制 MHCC97-H 细胞在体外的增殖、黏附、迁移和侵袭。这不仅是通过降低 AEG-1 和 N-钙黏蛋白的表达来实现的，还通过增强 E-钙黏蛋白的表达来实现的。因此，这些数据表明，HP 可以通过调节 AEG-1/EMT 通路来抑制 MHCC97-H 细胞的生长和转移潜能。
Zotero 插件