具有图案化细胞外基质和亚细胞硬度梯度的底物揭示了局部生物力学响应。
Substrates with patterned extracellular matrix and subcellular stiffness gradients reveal local biomechanical responses.
机构信息
Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza 5121 Engineering V, Los Angeles, CA, 90095, USA.
出版信息
Adv Mater. 2014 Feb 26;26(8):1242-7. doi: 10.1002/adma.201304607. Epub 2013 Dec 9.
Abstract
A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub-cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography-based fabrication is amenable to integration with complex microfabricated substructures.
摘要
开发了一种基底制造工艺，可在亚细胞空间分辨率下对细胞外基质（ECM）和刚性进行图案化。当在这些基底上培养细胞时，发现细胞在细胞骨架组装方面局部响应。所提出的方法允许深入了解机械信号的生物学解释，而基于光刻的制造方法则适合与复杂的微制造子结构集成。
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