一种自动化多重免疫荧光检测沙眼衣原体免疫球蛋白 G 的性能评估。

Performance of an automated multiplex immunofluorescence assay for detection of Chlamydia trachomatis immunoglobulin G.

机构信息

Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne and University Hospital Center (CHUV), Lausanne, Switzerland; Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.

Departments of Obstetrics and Gynecology, Materno-fetal and Obstetrics Research Unit, University Hospital Center (CHUV), Lausanne, Switzerland.

出版信息

Diagn Microbiol Infect Dis. 2014 Mar;78(3):217-9. doi: 10.1016/j.diagmicrobio.2013.11.022. Epub 2013 Nov 27.

DOI:10.1016/j.diagmicrobio.2013.11.022

PMID:24365033

Abstract

Chlamydia serology is indicated to investigate etiology of miscarriage, infertility, pelvic inflammatory disease, and ectopic pregnancy. Here, we assessed the reliability of a new automated-multiplex immunofluorescence assay (InoDiag test) to detect specific anti-C. trachomatis immunoglobulin G. Considering immunofluorescence assay (IF) as gold standard, InoDiag tests exhibited similar sensitivities (65.5%) but better specificities (95.1%-98%) than enzyme-linked immunosorbent assays (ELISAs). InoDiag tests demonstrated similar or lower cross-reactivity rates when compared to ELISA or IF.

摘要

沙眼衣原体血清学用于调查流产、不孕、盆腔炎和宫外孕的病因。在这里，我们评估了一种新的自动化多重免疫荧光检测（InoDiag 检测）用于检测特异性抗沙眼衣原体 IgG 的可靠性。以免疫荧光检测为金标准，InoDiag 检测显示出相似的敏感性（65.5%），但特异性更好（95.1%-98%），比酶联免疫吸附试验（ELISA）更好。与 ELISA 或 IF 相比，InoDiag 检测显示出相似或更低的交叉反应率。