伏安法对扁桃酸对映异构体的分辨。
Voltammetric discrimination of mandelic acid enantiomers.
机构信息
Science Institute, Selcuk University, 42003 Selcuklu, Konya, Turkey; Department of Science and Technology, Ahmet Kelesoglu Education Faculty, Necmettin Erbakan University, 42099 Meram, Konya, Turkey.
Department of Chemistry Education, Ahmet Kelesoglu Education Faculty, Necmettin Erbakan University, 42099 Meram, Konya, Turkey.
出版信息
Anal Biochem. 2014 Mar 15;449:83-9. doi: 10.1016/j.ab.2013.12.023. Epub 2013 Dec 27.
We report a novel electrochemical biosensor for direct discrimination of D- and L-mandelic acid (D- and L-MA) in aqueous medium. The glassy carbon electrode (GCE) surface was modified with reduced graphene oxide (rGO) and γ-globulin (GLOB). Electrochemical characterization of the modified electrodes was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The modified electrode surfaces were also characterized by scanning electron microscopy. Electrochemical response of the prepared electrode (GCE/rGO/GLOB) for discrimination of D- and L-MA enantiomers was investigated by cyclic voltammetry and was compared with bare GCE in the concentration range of 2 to 10 mM. Whereas the bare GCE showed no electrochemical response for the MA enantiomers, the GCE/rGO/GLOB electrode exhibited direct and selective discrimination with different oxidation potential values of 1.47 and 1.71 V and weak reduction peaks at potential values of -1.37 and -1.48 V, respectively. In addition, electrochemical performance of the modified electrode was investigated in mixed solution of D- and L-MA. The results show that the produced electrode can be used as electrochemical chiral biosensor for MA.
我们报告了一种用于在水溶液中直接区分 D-和 L-扁桃酸（D-和 L-MA）的新型电化学生物传感器。玻碳电极（GCE）表面用还原氧化石墨烯（rGO）和γ-球蛋白（GLOB）进行了修饰。通过循环伏安法和电化学阻抗谱研究了修饰电极的电化学特性。还通过扫描电子显微镜对修饰电极表面进行了表征。通过循环伏安法研究了制备的电极（GCE/rGO/GLOB）对 D-和 L-MA 对映体的区分电化学响应，并与裸 GCE 进行了比较，浓度范围为 2 至 10 mM。虽然裸 GCE 对 MA 对映体没有电化学响应，但 GCE/rGO/GLOB 电极表现出直接和选择性的区分，其氧化电位值分别为 1.47 和 1.71 V，以及在电位值为-1.37 和-1.48 V 时的弱还原峰。此外，还研究了修饰电极在 D-和 L-MA 混合溶液中的电化学性能。结果表明，所制备的电极可用作 MA 的电化学手性生物传感器。
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