采用基质辅助激光解吸/电离飞行时间质谱法的小分子分析用对二甲苯基质芯片。

Parylene-matrix chip for small molecule analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

机构信息

Department of Materials Sciences and Engineering, Yonsei University, 134 Shin-chon-dong, Seo-dae-mun-gu, Seoul, 120-749, Korea.

出版信息

Rapid Commun Mass Spectrom. 2014 Feb 15;28(3):274-80. doi: 10.1002/rcm.6782.

DOI:10.1002/rcm.6782

PMID:24375878

Abstract

RATIONALE

In matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are ionized, they generate unreproducible mass peaks such that MALDI-TOF MS is nearly impossible in the low mass-to-charge (m/z) range (<1000). In this work, we aimed to develop a parylene-matrix chip for the detection of small molecules in the low m/z range by using MALDI-TOF MS.

METHODS

The parylene-matrix chip was fabricated by the deposition of a partially porous parylene-N thin film on a dried organic matrix array. The properties of the parylene thin film were analyzed by atomic force microscopy (AFM) and cyclic voltammetry (CV). Mass spectrometry was performed by using a parylene-matrix chip with eight amino acids as model analytes.

RESULTS

The surface roughness and the electric conductivity of the parylene-N film were analyzed by AFM and CV analysis to determine its suitability for a parylene-matrix chip. The ionization of samples on the parylene-matrix chip was optimized by adjusting the laser intensity. The feasibility of applying a parylene-matrix chip for small molecule analysis was tested by using eight kinds of amino acids as model analytes and the simultaneous detection of multiple analytes from the amino acid mixture was also demonstrated.

CONCLUSIONS

The parylene-matrix chip can be applied for the detection of multiple analytes in the m/z ratio range of small molecules (<1000) using MALDI-TOF MS.

摘要

原理

在基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱（MS）中，已知分析物分子通过与有机基质分子混合而被离子化。随着有机基质分子被离子化，它们会产生不可重现的质量峰，使得 MALDI-TOF MS 在低质量电荷（m/z）范围内（<1000）几乎不可能进行。在这项工作中，我们旨在开发一种聚对二甲苯基质芯片，通过 MALDI-TOF MS 来检测低 m/z 范围内的小分子。

方法

聚对二甲苯基质芯片是通过在干燥的有机基质阵列上沉积部分多孔聚对二甲苯-N 薄膜来制造的。通过原子力显微镜（AFM）和循环伏安法（CV）分析聚对二甲苯薄膜的性质。使用带有八种氨基酸作为模型分析物的聚对二甲苯基质芯片进行质谱分析。

结果

通过 AFM 和 CV 分析对聚对二甲苯-N 薄膜的表面粗糙度和电导率进行了分析，以确定其是否适合用作聚对二甲苯基质芯片。通过调整激光强度优化了样品在聚对二甲苯基质芯片上的离子化。使用八种氨基酸作为模型分析物，并证明了从氨基酸混合物中同时检测多种分析物，验证了聚对二甲苯基质芯片在小分子分析中的应用可行性。