Xu Qian, Chen Lin-lin, Zhang Rui-hua, Yang Lei, Xie Zhi-jing, Zhu Yan-li, Jiang Shi-jin, Si Xing-kui
College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Department of Animal Medicine, Foshan Science and Technology University, Foshan 528231, China.
Bing Du Xue Bao. 2013 Sep;29(5):522-8.
To reveal the genetic variation of the viral protein 1 (VP1) gene of the duck hepatitis A virus type 3 (DHAV-3), the VP1 gene of 13 virulent DHAV-3 strains isolated from Shandong province of China in 2012 were amplified by RT-PCR, sequenced and analyzed. The results showed that all the VP1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94. 6%-99.9% and amino acid identities of 95.0%-100%. The nucleotide and amino acid sequence homologies between the 13 DHAV-3 isolates and other 31 DHAV-3 reference strains were 92.5%-100% and 90. 8%-100%, respectively. Phylogenetic analysis showed that the VP1 gene of DHAV-3 had distinct geographical characteristics. Distribution of genotypes of the 44 DHAV-3 strains was as follows: except the vaccine strain B63, all the other Chinese isolates belonged to genotype I (GI), Vietnamese wild isolates mainly belonged to subtype 1 (S1) of genotype II (GII), and all Korean isolates belonged to subtype 2 (S2) of GII.
为揭示鸭甲型肝炎病毒3型（DHAV-3）病毒蛋白1（VP1）基因的遗传变异情况，对2012年从中国山东省分离的13株强毒株DHAV-3的VP1基因进行RT-PCR扩增、测序及分析。结果显示，13株分离株的VP1基因均含720个核苷酸，编码240个氨基酸，核苷酸同源性为94.6%-99.9%，氨基酸同源性为95.0%-100%。13株DHAV-3分离株与其他31株DHAV-3参考毒株的核苷酸和氨基酸序列同源性分别为92.5%-100%和90.8%-100%。系统发育分析表明，DHAV-3的VP1基因具有明显的地理特征。44株DHAV-3毒株的基因型分布如下：除疫苗株B63外，其他中国分离株均属于基因型I（GI），越南野生分离株主要属于基因型II（GII）的亚型1（S1），韩国分离株均属于GII的亚型2（S2）。
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