Marchiaro Patricia M, Brambilla Luciano, Morán-Barrio Jorgelina, Revale Santiago, Pasteran Fernando, Vila Alejandro J, Viale Alejandro M, Limansky Adriana S
Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina.
Antimicrob Agents Chemother. 2014;58(3):1816-21. doi: 10.1128/AAC.02494-13. Epub 2014 Jan 6.
The complete sequence of the carbapenem-resistance-conferring conjugative plasmid pLD209 from a Pseudomonas putida clinical strain is presented. pLD209 is formed by 3 well-defined regions: an adaptability module encompassing a Tn402-like class 1 integron of clinical origin containing blaVIM-2 and aacA4 gene cassettes, partitioning and transfer modules, and a replication module derived from plasmids of environmental bacteria. pLD209 is thus a mosaic of modules originating in both the clinical and environmental (nonclinical) microbiota.
本文报道了来自恶臭假单胞菌临床菌株的携带碳青霉烯抗性的接合质粒pLD209的完整序列。pLD209由3个明确的区域组成:一个适应性模块,包含一个临床来源的Tn402样1类整合子,其中含有blaVIM-2和aacA4基因盒;分区和转移模块;以及一个源自环境细菌质粒的复制模块。因此,pLD209是一个源自临床和环境(非临床)微生物群的模块镶嵌体。