Department of Medicine, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Department of Medicine, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Biol Blood Marrow Transplant. 2014 Apr;20(4):450-62. doi: 10.1016/j.bbmt.2014.01.002. Epub 2014 Jan 6.
Graft-versus-host disease (GVHD) is a major transplantation complication. The purpose of this study was to measure immune cell subsets by flow cytometry early after transplantation (before median day of GVHD onset) to identify subsets that may play a role in GVHD pathogenesis. We also measured the subsets later after transplantation to determine which subsets may be influenced by GVHD or its treatment. We studied 219 patients. We found that acute GVHD (aGVHD) was preceded by high counts of CD4 T cells and CD8 T cells. It was followed by low counts of total and naive B cells, total and cytolytic NK cells, and myeloid and plasmacytoid dendritic cells. Chronic GVHD (cGVHD) was preceded by low counts of memory B cells. In conclusion, both CD4 and CD8 T cells appear to play a role in the pathogenesis of aGVHD. Generation of B cells, NK cells, and dendritic cells may be hampered by aGVHD and/or its treatment. Memory B cells may inhibit the development of cGVHD.
移植物抗宿主病（GVHD）是一种主要的移植并发症。本研究的目的是通过流式细胞术在移植后早期（GVHD 发病中位数之前）测量免疫细胞亚群，以确定可能在 GVHD 发病机制中起作用的亚群。我们还在移植后较晚时测量了这些亚群，以确定哪些亚群可能受到 GVHD 或其治疗的影响。我们研究了 219 名患者。我们发现，急性移植物抗宿主病（aGVHD）之前 CD4 T 细胞和 CD8 T 细胞计数较高。之后，总 B 细胞和幼稚 B 细胞、总 NK 细胞和细胞毒性 NK 细胞以及髓系和浆细胞样树突状细胞计数较低。慢性移植物抗宿主病（cGVHD）之前记忆 B 细胞计数较低。总之，CD4 和 CD8 T 细胞似乎都在 aGVHD 的发病机制中起作用。B 细胞、NK 细胞和树突状细胞的生成可能受到 aGVHD 和/或其治疗的阻碍。记忆 B 细胞可能抑制 cGVHD 的发展。
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