RhoC参与神经干细胞/祖细胞的迁移。
RhoC involved in the migration of neural stem/progenitor cells.
作者信息
Zhang Can, Ge Xuemei, Lok Kenghoe, Zhao Lu, Yin Ming, Wang Ze-Jian
机构信息
School of Pharmacy, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China.
出版信息
Cell Mol Neurobiol. 2014 Apr;34(3):409-17. doi: 10.1007/s10571-014-0026-0. Epub 2014 Jan 11.
Alzheimer's disease (AD) is characterized by deposition of beta-amyloid peptides (Aβ) and progressive loss of neurons. Neural stem/progenitor cells (NSPCs) can proliferate and produce immature neurons even in the brain of AD patients. However, Aβ42 significantly decreased the expression of RhoC in NSPCs during the co-incubation (P < 0.01). Treating with RhoC siRNA prevented membrane from protrusion and led to a significant reduction in cell migration in responses to SDF-1. Compared with wild-type mice, the numbers of RhoC-immunoreactive cells in hippocampus and cortex were significantly down-regulated in APP/PS1 mice aged 9 months. The results suggest that Aβ42 down-regulates the expression of RhoC in NSPCs in vitro and in vivo; down-regulated RhoC expression results in decreased migration of NSPCs.
阿尔茨海默病（AD）的特征是β-淀粉样肽（Aβ）沉积和神经元的进行性丧失。即使在AD患者的大脑中，神经干/祖细胞（NSPCs）也能增殖并产生未成熟的神经元。然而，在共孵育期间，Aβ42显著降低了NSPCs中RhoC的表达（P<0.01）。用RhoC小干扰RNA处理可防止细胞膜突出，并导致对基质细胞衍生因子-1（SDF-1）反应的细胞迁移显著减少。与野生型小鼠相比，9月龄APP/PS1小鼠海马和皮质中RhoC免疫反应性细胞的数量显著下调。结果表明，Aβ42在体外和体内均下调NSPCs中RhoC的表达；RhoC表达下调导致NSPCs迁移减少。
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