从环境样品中分离的真菌产木聚糖酶和纤维素酶的筛选。
Screening of fungi isolated from environmental samples for xylanase and cellulase production.
作者信息
Ja'afaru Mohammed Inuwa
机构信息
Department of Microbiology, Modibbo Adama University of Technology, PMB 2076, Yola 64001, Nigeria.
出版信息
ISRN Microbiol. 2013 Dec 12;2013:283423. doi: 10.1155/2013/283423. eCollection 2013.
Abstract
The aim of this work is to select filamentous fungal strains isolated from saw dust, soil, and decaying wood with the potential to produce xylanase and cellulase enzymes. A total of 110 fungi were isolated. Fifty-seven (57) of these fungi were isolated from soil samples, 32 from sawdust, and 19 from decaying wood. Trichoderma and Aspergillus had the highest relative occurrence of 42.6% and 40.8%, respectively. Trichoderma viride Fd18 showed the highest specific activity of 1.30 U mg(-1) protein for xylanase, while the highest cellulase activity of 1.23 U mg(-1) was shown by Trichoderma sp. F4. The isolated fungi demonstrated potential for synthesizing the hydrolytic enzymes.
摘要
这项工作的目的是从锯末、土壤和腐朽木材中筛选出具有产生木聚糖酶和纤维素酶潜力的丝状真菌菌株。共分离出110株真菌。其中57株真菌从土壤样品中分离得到，32株从锯末中分离得到，19株从腐朽木材中分离得到。木霉属和曲霉属的相对出现率最高，分别为42.6%和40.8%。绿色木霉Fd18的木聚糖酶比活性最高，为1.30 U mg(-1)蛋白质，而木霉属F4的纤维素酶活性最高，为1.23 U mg(-1)。分离出的真菌显示出合成水解酶的潜力。
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