Wang Wei, Jiang Hong-mei, Li Wei-ren, Zuo Lu, Tao Lei, Yu Xiang
Department of Burns & Plastic Surgery, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China.
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Zhonghua Yi Xue Za Zhi. 2013 Nov 12;93(42):3394-7.
To explore the effects of low-dose insulin on oxidation-reduction of heart and kidney in rats immediately after severe burns.
Twenty four male Sprague-Dawley (SD) rats were randomly divided into 3 groups of sham burn, burn and treatment (n = 8 each). The sham burn group was placed into 37 °C warm water for 15 seconds to simulate burn process and received no fluid replacement. The burn and treatment groups were immersed into (95 ± 0.5) °C hot water for 15 seconds to make a rat model of 30% total burn surface area, III degree burn injury and immediately received an intraperitoneal injection of physiological saline (40 ml/kg). At the same time, a subcutaneous injection of insulin (1.0 U×kg(-1)×d(-1)) was given in the treatment group and a subcutaneous injection of same-volume physiological saline in the burn group. The rats were sacrificed after 24 post-scald hours (PSH). Abdominal aortic blood was collected for an analysis of blood glucose. The oxidation and antioxidation parameters of heart and kidney, such as malondialdehyde (MDA), xanthine oxidase (XO), myeloperoxidase (MPO), total superoxide dismutase (T-SOD), superoxide dismutase 1, 2 (SOD1, 2), catalase (CAT) and glutathione peroxidase (GPx), were detected by spectrophotometry.
Compared with the sham burn group, MDA content, XO and MPO activities of heart and kidney were significantly higher in the burn group (all P < 0.05); in the treatment group, MDA content and XO activity of heart were significantly lower than the burn group ((0.85 ± 0.07) vs (1.11 ± 0.07) nmol/mg, (69.72 ± 1.94) vs (77.21 ± 2.10) U/g) while the MPO activities of heart and kidney were significantly lower (all P < 0.05).compared with the sham burn group, the activities of T-SOD, CAT, GPx of heart and kidney were significantly lower in the burn group, SOD1 activity of kidney was significantly lower, but SOD2 activity of kidney was significantly higher while SOD2 activity of heart was significantly lower (all P < 0.05); Compared with the burn group, the activities of T-SOD and SOD1 of heart in the treatment group were significantly higher ((83.5 ± 2.5) vs (79.6 ± 3.2), (62.8 ± 2.3) vs (58.8 ± 3.0) U/mg), CAT and GPx activity of heart and kidney were significantly higher (all P < 0.05). Compared with the sham burn group, blood glucose in the burn and treatment group were significantly higher ((7.81 ± 0.30), (7.19 ± 0.22) vs (6.30 ± 0.24) mmol/L) and blood glucose in the treatment group was significantly lower than the burn group((7.81 ± 0.30) mmol/L)(all P < 0.05).
During an early stage, a low-dose insulin may intervene in heart tissue lipid peroxidation of severely burned rats. And differences exist in the effects of oxidation-reduction between heart and kidney.
探讨小剂量胰岛素对严重烧伤大鼠伤后即刻心、肾氧化还原状态的影响。
将24只雄性Sprague-Dawley（SD）大鼠随机分为假烧伤组、烧伤组和治疗组，每组8只。假烧伤组大鼠置于37℃温水中15秒以模拟烧伤过程，不进行液体复苏。烧伤组和治疗组大鼠浸入（95±0.5）℃热水中15秒，制成30%总体表面积Ⅲ度烧伤大鼠模型，伤后即刻腹腔注射生理盐水（40 ml/kg）。同时，治疗组皮下注射胰岛素（1.0 U×kg⁻¹×d⁻¹），烧伤组皮下注射等体积生理盐水。伤后24小时处死大鼠，采集腹主动脉血检测血糖。采用分光光度法检测心、肾组织中丙二醛（MDA）、黄嘌呤氧化酶（XO）、髓过氧化物酶（MPO）、总超氧化物歧化酶（T-SOD）、超氧化物歧化酶1、2（SOD1、2）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）等氧化及抗氧化指标。
与假烧伤组比较，烧伤组心、肾组织MDA含量、XO及MPO活性均显著升高（均P<0.05）；治疗组心组织MDA含量及XO活性显著低于烧伤组（（0.85±0.07） vs （1.11±0.07） nmol/mg，（69.72±1.94） vs （77.21±2.10） U/g），心、肾组织MPO活性均显著降低（均P<0.05）。与假烧伤组比较，烧伤组心、肾组织T-SOD、CAT、GPx活性均显著降低，肾组织SOD1活性显著降低，肾组织SOD2活性显著升高，心组织SOD2活性显著降低（均P<0.05）；与烧伤组比较，治疗组心组织T-SOD及SOD1活性显著升高（（83.5±2.5） vs （79.6±3.2），（62.8±2.3） vs （58.8±3.0） U/mg），心、肾组织CAT及GPx活性均显著升高（均P<0.05）。与假烧伤组比较，烧伤组和治疗组血糖均显著升高（（7.81±0.30），（7.19±0.22） vs （6.30±0.24） mmol/L），治疗组血糖显著低于烧伤组（（7.81±0.30） mmol/L）（均P<0.05）。
伤后早期小剂量胰岛素可干预严重烧伤大鼠心脏组织脂质过氧化反应。心、肾组织氧化还原状态变化存在差异。
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