用于功能解析的糖蛋白的化学合成与化学酶法合成
Chemical and chemoenzymatic synthesis of glycoproteins for deciphering functions.
作者信息
Wang Lai-Xi, Amin Mohammed N
机构信息
Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Institute of Human Virology and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
出版信息
Chem Biol. 2014 Jan 16;21(1):51-66. doi: 10.1016/j.chembiol.2014.01.001.
Glycoproteins are an important class of biomolecules involved in a number of biological recognition processes. However, natural and recombinant glycoproteins are usually produced as mixtures of glycoforms that differ in the structures of the pendent glycans, which are difficult to separate in pure glycoforms. As a result, synthetic homogeneous glycopeptides and glycoproteins have become indispensable probes for detailed structural and functional studies. A number of elegant chemical and biological strategies have been developed for synthetic construction of tailor-made, full-size glycoproteins to address specific biological problems. In this review, we highlight recent advances in chemical and chemoenzymatic synthesis of homogeneous glycoproteins. Selected examples are given to demonstrate the applications of tailor-made, glycan-defined glycoproteins for deciphering glycosylation functions.
糖蛋白是一类重要的生物分子，参与多种生物识别过程。然而，天然和重组糖蛋白通常以糖型混合物的形式产生，这些糖型在侧链聚糖结构上存在差异，很难分离成纯糖型。因此，合成的均一糖肽和糖蛋白已成为详细结构和功能研究中不可或缺的探针。为了合成定制的全尺寸糖蛋白以解决特定的生物学问题，人们已经开发了许多精妙的化学和生物学策略。在这篇综述中，我们重点介绍了均一糖蛋白化学合成和化学酶促合成方面的最新进展。通过列举一些实例来展示定制的、聚糖定义的糖蛋白在解析糖基化功能方面的应用。
Zotero 插件