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运动疗法对大鼠缺血性损伤及再灌注的影响。

Effects of kinesiotherapy in ischemic lesion and reperfusion in rats.

作者信息

Moscardini Flavia, Barbosa Everton Horiquini, Garcia Estela Fagionato, Borges Ana Paula Oliveira, Bachur José Alexandre, Quemelo Paulo Roberto Veiga

机构信息

Universidade de Franca, Franca - SP, Brazil.

出版信息

Acta Ortop Bras. 2012;20(3):131-5. doi: 10.1590/S1413-78522012000300001.

Abstract

OBJECTIVE

To investigate the effect of kinesiotherapy on the functionality of the pelvic limb of rats after ischemic and reperfusion injury.

METHODS

10 rats were divided into two groups, GI (control) and GII (kinesiotherapy). All the animals underwent ischemia for a period of three hours, followed by tissue reperfusion. In Group GII, non-resistive systemic kinesiotherapy was performed (swimming) in three weekly sessions of 50 minutes, over a period of four weeks, while the GI animals remained at rest. Functional analysis of motor behavior was evaluated weekly. The animals were then sacrificed, and the soleus and gastrocnemius muscles and the sciatic nerve removed for histopathological analysis.

RESULTS

There was a significant recovery of motor behavior with kinesiotherapeutic treatment during the four weeks of treatment. However, the histological examination of the tissues showed no morphological changes of cell injury and repair.

CONCLUSION

It was not possible to affirm that the exercise was effective in cell repair, because neither of the groups (control and experimental) showed any histological difference. On the other hand, systemic kinesiotherapy showed a beneficial effect on functional rehabilitation after ischemia and reperfusion. Level of evidence III, Case-Control Study .

摘要

目的

探讨运动疗法对大鼠缺血再灌注损伤后后肢功能的影响。

方法

将10只大鼠分为两组,GI组(对照组)和GII组(运动疗法组)。所有动物均经历3小时的缺血,随后进行组织再灌注。在GII组中,进行非抵抗性全身运动疗法(游泳),每周3次,每次50分钟,持续4周,而GI组动物保持静止。每周评估运动行为的功能分析。然后处死动物,取出比目鱼肌、腓肠肌和坐骨神经进行组织病理学分析。

结果

在治疗的四周内,运动疗法治疗使运动行为有显著恢复。然而,组织的组织学检查未显示细胞损伤和修复的形态学变化。

结论

无法确定运动对细胞修复有效,因为两组(对照组和实验组)均未显示出任何组织学差异。另一方面,全身运动疗法对缺血再灌注后的功能康复显示出有益效果。证据级别III,病例对照研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e00/3718438/871592336857/aob-20-131-g01.jpg

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