锌对接种抗球虫病疫苗的肉鸡体液免疫和细胞介导免疫的影响。
Effect of zinc on humoral and cell-mediated immunity of broilers vaccinated against coccidiosis.
作者信息
Sajadifar Sobhan, Miranzadeh Hadi, Moazeni Milad
机构信息
Department of Epidemiology and Parasitology, Faculty of Veterinary Medicine and Zootechnics, Armenian National Agrarian University, Yerevan, Armenia.
Department of Veterinary, University of Applied Science and Technology, Institute of Science Applied Higher Education of Jahad -e- Agriculture, Isfahan, Iran.
出版信息
Iran J Parasitol. 2013 Jul;8(3):474-80.
BACKGROUND
The aim of the present study was the comparison of humoral and cell-mediated immunity in broilers fed with different levels of zinc during a coccidiosis challenge.
METHODS
One hundred and forty-four one-day-old broiler chicks were used with three dietary zinc (40, 120 and 200 mg/kg). At 14 d of age, all birds were inoculated orally with 5×10(3) sporulated oocysts of E. Tenella. At 2, 22, 32, 42 days of age, the blood serums were tested for antibody titer against Newcastle disease vaccine, using the standard HI test. On day 42 the sum of nitrite and nitrate based on the reduction of nitrate to nitrite by cadmium and white blood cell count (WBC) using a hemocytometer were measured.
RESULTS
At 42 d, levels of 120 and 200 mg significantly (P< 0.05) increased the antibody titer in compare with the control. The peak response of CBH was observed at the level of 200 mg Zn/kg diet. Also both level of 120 and 200 mg Zn/kg diet increased WBC count and sum of nitrite and nitrate in serum compared with the control.
CONCLUSION
The levels of 120 and 200 mg Zn/kg diet could be considered as a non-pharmacologic booster of immunity in broilers chicks infected with E. Tenella.
背景
本研究的目的是比较在球虫病攻击期间饲喂不同锌水平的肉鸡的体液免疫和细胞介导免疫。
方法
使用144只1日龄的肉鸡雏鸡，采用三种日粮锌水平（40、120和200毫克/千克）。在14日龄时，所有鸡只口服接种5×10³个柔嫩艾美耳球虫的孢子化卵囊。在2、22、32、42日龄时，使用标准血凝抑制试验检测血清中针对新城疫疫苗的抗体效价。在42日龄时，通过镉将硝酸盐还原为亚硝酸盐来测定亚硝酸盐和硝酸盐的总量，并使用血细胞计数器测量白细胞计数（WBC）。
结果
在42日龄时，与对照组相比，120和200毫克水平显著（P<0.05）提高了抗体效价。在日粮锌水平为200毫克/千克时观察到细胞介导免疫的峰值反应。此外，与对照组相比，日粮锌水平为120和200毫克/千克时均增加了血清中的白细胞计数以及亚硝酸盐和硝酸盐的总量。
结论
日粮锌水平为120和200毫克/千克可被视为感染柔嫩艾美耳球虫的肉鸡雏鸡的非药物免疫增强剂。
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