来自鱿鱼巨大轴突的单个钠通道。
Single sodium channels from the squid giant axon.
作者信息
Bezanilla F
机构信息
Department of Physiology, Jerry Lewis Neuromuscular Research Center, Ahmanson Laboratory of Neurobiology, University of California, Los Angeles 90024.
出版信息
Biophys J. 1987 Dec;52(6):1087-90. doi: 10.1016/S0006-3495(87)83304-0.
Since the work of A. L. Hodgkin and A. F. Huxley (1952. J. Physiol. [Lond.].117:500-544) the squid giant axon has been considered the classical preparation for the study of voltage-dependent sodium and potassium channels. In this preparation much data have been gathered on macroscopic and gating currents but no single sodium channel data have been available. This paper reports patch clamp recording of single sodium channel events from the cut-open squid axon. It is shown that the single channel conductance in the absence of external divalent ions is approximately 14 pS, similar to sodium channels recorded from other preparations, and that their kinetic properties are consistent with previous results on gating and macroscopic currents obtained from the perfused squid axon preparation.
自A. L. 霍奇金和A. F. 赫胥黎1952年的研究工作（《生理学杂志》[伦敦]，117卷：500 - 544页）以来，枪乌贼巨大轴突一直被视为研究电压依赖性钠通道和钾通道的经典标本。在这个标本上，已经收集了大量关于宏观电流和门控电流的数据，但尚无单个钠通道的数据。本文报道了对切开的枪乌贼轴突进行单钠通道事件的膜片钳记录。结果表明，在没有外部二价离子的情况下，单通道电导约为14皮西门子，与从其他标本记录到的钠通道相似，并且它们的动力学特性与先前从灌注枪乌贼轴突标本获得的门控电流和宏观电流的结果一致。
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