Graduate Institute of Clinical Medical Science, China Medical University, 91 Hsuehshih Road, Taichung 40402, Taiwan.
Department of Chemistry, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan.
J Biosci Bioeng. 2014 Jul;118(1):88-93. doi: 10.1016/j.jbiosc.2014.01.001. Epub 2014 Feb 3.
Phellinus linteus and its constituent hispolon induce potent anti-inflammatory activity in macrophages. Efficient production of the effective constituent and the biological function of P. linteus in the regulation of innate sensing have rarely been investigated. The aim of this study was to efficiently manufacture P. linteus-fermented broth containing the effective constituent, hispolon, and evaluate its immunoregulatory functions in macrophages. Four distinct fermented broths (PL1-4) and the medium dialyzate (MD) were prepared to screen suitable culture conditions for the mycelial growth of P. linteus. The P. linteus-fermented broth exhibited a dose-responsive inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production by murine macrophages. In addition, the P. linteus-fermented broths suppressed macrophage LPS-mediated nuclear factor (NF)-κB activity and tumor necrosis factor (TNF)-α. Among the tested samples from P. linteus, PL4 contained vast amounts of hispolon and showed the greatest anti-inflammatory activity in both the RAW264.7 cells and murine primary peritoneal exudate macrophages (PEMs). This study demonstrates that the purification of the effective constituent from P. linteus-fermented broth may enable the production of a potent therapeutic agent for anti-inflammation in macrophages.
云芝及其组成部分香菇素有很强的抗炎活性。其有效成分的高效生产以及云芝在先天感应调节中的生物学功能很少被研究。本研究的目的是高效生产含有有效成分香菇素的云芝发酵液，并评估其在巨噬细胞中的免疫调节功能。制备了四种不同的发酵液（PL1-4）和培养基透析液（MD），以筛选适合云芝菌丝体生长的培养条件。云芝发酵液对脂多糖（LPS）诱导的小鼠巨噬细胞一氧化氮（NO）的产生具有剂量依赖性抑制作用。此外，云芝发酵液还抑制了巨噬细胞脂多糖介导的核因子（NF）-κB 活性和肿瘤坏死因子（TNF）-α。在所测试的云芝样本中，PL4 含有大量的香菇素，在 RAW264.7 细胞和小鼠原代腹腔渗出巨噬细胞（PEMs）中均表现出最强的抗炎活性。本研究表明，从云芝发酵液中纯化有效成分可能能够生产出用于巨噬细胞抗炎的有效治疗药物。
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