Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
Division of Food Bioscience, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
Carbohydr Polym. 2014 Feb 15;102:185-91. doi: 10.1016/j.carbpol.2013.11.022. Epub 2013 Nov 27.
Fucoidan, extracted from Ecklonia cava, has been extensively studied because of its wide biological activities. However, antioxidative activities have not been yet examined. Therefore we evaluated in vitro and in vivo studies on antioxidative activities of E. cava fucoidan (ECF). ECF exhibited more prominent effects in peroxyl radical scavenging activity, compared to the other scavenging activities. Thus, ECF was further evaluated for its protective ability against 2,2'-azobis dihydrochloride induced oxidative stress in Vero cells and ECF strongly reduced the AAPH-induced oxidative damage through scavenging intracellular reactive oxygen species. Furthermore, we evaluated protective effect of ECF against AAPH-induced oxidative stress in zebrafish model. ECF significantly reduced ROS generation, lipid peroxidation and cell death in zebrafish model. These findings indicate that ECF has antioxidant activities in vitro Vero cells and in vivo zebrafish model, even though ECF is not a polyphenol or flavonoid compound and does not contain benzene rings or conjugated structures.
岩藻聚糖硫酸酯是从裙带菜中提取的，由于其广泛的生物活性而受到广泛研究。然而，其抗氧化活性尚未得到检验。因此，我们评估了裙带菜岩藻聚糖硫酸酯（ECF）的体外和体内抗氧化活性。与其他清除活性相比，ECF 在过氧自由基清除活性方面表现出更显著的效果。因此，进一步评估了 ECF 对 2,2'-偶氮二盐酸（AAPH）诱导的 Vero 细胞氧化应激的保护能力，并且 ECF 通过清除细胞内活性氧来强烈减少 AAPH 诱导的氧化损伤。此外，我们评估了 ECF 在斑马鱼模型中对 AAPH 诱导的氧化应激的保护作用。ECF 可显著减少斑马鱼模型中的 ROS 生成、脂质过氧化和细胞死亡。这些发现表明，即使 ECF 不是多酚或类黄酮化合物，也不含有苯环或共轭结构，它在体外 Vero 细胞和体内斑马鱼模型中具有抗氧化活性。
