Pelton B K, North M, Palmer R G, Hylton W, Smith-Burchnell C, Sinclair A L, Malkovsky M, Dalgleish A G, Denman A M
Connective Tissue Diseases Research Group, Clinical Research Centre, Harrow, Middlesex.
Ann Rheum Dis. 1988 Mar;47(3):206-9. doi: 10.1136/ard.47.3.206.
Evidence for retroviral infection in general and human immunodeficiency virus (HIV) infection in particular was sought in freshly isolated peripheral blood T cells, B cells, and monocyte-macrophages from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) and also in T cell and B cell lines established from the same source. Similar cells isolated from rheumatoid synovial membrane were also examined. The strategy used for the detection of virus was cocultivation with susceptible cell lines looking for syncytia formation, reverse transcriptase production, and nucleic acid hybridisation with HIV cDNA probes. No evidence for infection was obtained.
研究人员在类风湿性关节炎（RA）和系统性红斑狼疮（SLE）患者新鲜分离的外周血T细胞、B细胞以及单核细胞-巨噬细胞中，尤其在从相同来源建立的T细胞和B细胞系中，寻找一般逆转录病毒感染特别是人类免疫缺陷病毒（HIV）感染的证据。同时也检测了从类风湿滑膜分离出的类似细胞。检测病毒的策略是与易感细胞系共培养，寻找多核巨细胞形成、逆转录酶产生以及与HIV cDNA探针的核酸杂交情况。未获得感染的证据。
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