Romariz Simone A, Garcia Karina de O, Paiva Daisyléa de Souza, Bittencourt Simone, Covolan Luciene, Mello Luis Eugênio, Longo Beatriz Monteiro
Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
Department of Physiology, Federal University of São Paulo, Rua Botucatu, 862, 04023-062 São Paulo, SP, Brazil.
Seizure. 2014 May;23(5):386-9. doi: 10.1016/j.seizure.2014.01.017. Epub 2014 Jan 30.
Diseases such as temporal lobe epilepsy, brain trauma and stroke can induce endothelial cell proliferation and angiogenesis in specific brain areas. During status epilepticus (SE), bone marrow-derived cells are able to infiltrate and proliferate, dramatically increasing at the site of injury. However, it is still unclear whether these cells directly participate in vascular changes induced by SE.
To investigate the possible role of bone marrow-derived cells in angiogenesis after seizures, we induced SE by pilocarpine injection in previously prepared chimeric mice. Mice were euthanized at 8h, 7d or 15d after SE onset.
Our results indicated that SE modified hippocampal vascularization and induced angiogenesis. Further, bone marrow-derived GFP(+) cells penetrated through the parenchyma and participated in the formation of new vessels after SE. We detected bone marrow-derived cells closely associated with vessels in the hippocampus, increasing the density of blood vessels that had decreased immediately after pilocarpine-induced SE.
We conclude that epileptic seizures directly affect vascularization in the hippocampus mediated by bone marrow-derived cells in a time-dependent manner.
颞叶癫痫、脑外伤和中风等疾病可诱导特定脑区的内皮细胞增殖和血管生成。在癫痫持续状态（SE）期间，骨髓来源的细胞能够浸润和增殖，在损伤部位显著增加。然而，这些细胞是否直接参与SE诱导的血管变化仍不清楚。
为了研究骨髓来源的细胞在癫痫发作后血管生成中的可能作用，我们通过在预先制备的嵌合小鼠中注射匹鲁卡品诱导SE。在SE发作后8小时、7天或15天对小鼠实施安乐死。
我们的结果表明，SE改变了海马血管化并诱导了血管生成。此外，骨髓来源的绿色荧光蛋白（GFP）阳性细胞穿透实质并在SE后参与新血管的形成。我们在海马中检测到与血管密切相关的骨髓来源的细胞，增加了匹鲁卡品诱导SE后立即减少的血管密度。
我们得出结论，癫痫发作以时间依赖性方式直接影响由骨髓来源的细胞介导的海马血管化。
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