结肠微穿孔：用于复制黏膜热损伤的大鼠动物模型。
Microperforation of the colon: animal model in rats to reproduce mucosal thermal damage.
机构信息
Endoscopy Unit, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Barcelona, Spain.
Endoscopy Unit, Department of Gastroenterology, Hospital Universitari Germans Trias i Pujol, Badalona, Spain.
出版信息
J Surg Res. 2014 May 15;188(2):415-8. doi: 10.1016/j.jss.2014.01.039. Epub 2014 Jan 29.
BACKGROUND
The aim of the present study was to develop a rat model of colonic microperforation secondary to thermal injury for future studies to assess new treatments.
METHODS
Twenty-four male Sprague-Dawley rats were used in this study. Hot biopsy forceps were used for all treatments. All lesions were created in proximal left colon using the soft coagulation setting. The power setting tested was 40 W, and the durations of monopolar soft coagulation application evaluated were 2, 3, and 4 s.
RESULTS
In the acute phase, 48 h after thermal injury, durations of cautery of 2 and 3 s resulted in transmural necrosis, whereas with 4 s microperforation was obtained. In the late phase, 7 d after the damage, only duration of cautery of 4 s showed deep cautery effects, with signs of peritonitis.
CONCLUSIONS
We determined optimal power settings and duration of therapy in a rat model for producing electrocautery that involves transmural necrosis with microperforation.
背景
本研究旨在建立一种因热损伤导致结肠微穿孔的大鼠模型，以便未来研究评估新的治疗方法。
方法
本研究使用了 24 只雄性 Sprague-Dawley 大鼠。所有治疗均使用热活检夹。所有损伤均采用近端左结肠软凝固设置进行。测试的功率设置为 40W，评估的单极软凝固应用时间分别为 2、3 和 4s。
结果
在急性阶段，热损伤后 48h，烧灼 2s 和 3s 导致全层坏死，而 4s 则导致微穿孔。在晚期，损伤后 7d，只有烧灼 4s 的时间显示出深烧灼效应，伴有腹膜炎迹象。
结论
我们在大鼠模型中确定了产生电灼的最佳功率设置和治疗时间，该模型涉及伴有微穿孔的全层坏死。
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