骨碎补富含黄酮提取物的化学成分及生物活性
Chemical composition and bioactivities of flavonoids-rich extract from Davallia cylindrica Ching.
作者信息
Cao Jianguo, Xia Xian, Dai Xiling, Wang Quanxi, Xiao Jianbo
机构信息
Department of Biology, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China.
Department of Biology, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China; Research Center of Engineering Technology for Extraction of Bioactive Compounds, Anhui Academy of Applied Technology, Suixi Road 312, 230031 Hefei, Anhui, PR China.
出版信息
Environ Toxicol Pharmacol. 2014 Mar;37(2):571-9. doi: 10.1016/j.etap.2014.01.011. Epub 2014 Jan 28.
The flavonoids profiles and bioactivities of flavonoids-rich extract from Davallia cylindrica Ching were investigated. The total flavonoids content in D. cylindrica was determined as about 164.41 mg/g. The main flavonoids in D. cylindrica were tentatively identified as quercetin-3-O-rutinoside, quercetin 7-O-glucoside, quercetin 7-O-glucoside, kaempferol 3-O-rutinoside, and quercitrin by UV and ESI-MS spectra. Flavonoids-rich extract (0.258 mg/ml) from D. cylindrica showed similar or higher free radical (O2(-), DPPH and ABTS) scavenging potential with that of rutin (0.25 mg/ml). The reducing power of flavonoids-rich extract (0.258 mg/ml) was slightly stronger than that of 0.25mg/ml rutin. The flavonoids extract from D. cylindrica exhibited cytotoxic effects on A549 cells. It exhibited a dose-dependent inhibition against acetylcholinesterase.
对骨碎补富含黄酮提取物的黄酮类成分及其生物活性进行了研究。骨碎补中总黄酮含量测定约为164.41mg/g。通过紫外光谱和电喷雾电离质谱对骨碎补中的主要黄酮类成分进行了初步鉴定，分别为槲皮素-3-O-芸香糖苷、槲皮素7-O-葡萄糖苷、山柰酚3-O-芸香糖苷和槲皮苷。骨碎补富含黄酮提取物（0.258mg/ml）与芦丁（0.25mg/ml）具有相似或更高的清除自由基（超氧阴离子、二苯基苦味酰基自由基和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子自由基）的能力。骨碎补富含黄酮提取物（0.258mg/ml）的还原能力略强于0.25mg/ml芦丁。骨碎补黄酮提取物对A549细胞具有细胞毒性作用。它对乙酰胆碱酯酶表现出剂量依赖性抑制作用。
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