利什曼原虫的线粒体半胱天冬酶：一种精氨酸特异性肽酶。

Leishmania metacaspase: an arginine-specific peptidase.

作者信息

Martin Ricardo, Gonzalez Iveth, Fasel Nicolas

机构信息

Department of Biochemistry, University of Lausanne, Lausanne, Switzerland.

出版信息

Methods Mol Biol. 2014;1133:189-202. doi: 10.1007/978-1-4939-0357-3_12.

DOI:10.1007/978-1-4939-0357-3_12

PMID:24567103

Abstract

The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.

摘要

本章旨在深入探讨利什曼原虫寄生虫的metacaspase作为精氨酸特异性半胱氨酸肽酶的情况。metacaspase在利什曼原虫中的生理作用仍存在争议，但其肽酶的酶活性已得到充分表征。在不同的可能表达系统中，缺乏metacaspase的酵母细胞（Δyca1）有助于研究硕大利什曼原虫metacaspase（LmjMCA）的活性。在此，我们描述了LmjMCA的纯化技术及其活性测定方法，为进一步鉴定LmjMCA底物提供了一个平台。