Culture of an epithelial cell line (MDCK) with a serum substitute.

This paper reports the suitability of culturing a line of dog kidney epithelial cells, MDCK, in the presence of a serum substitute, Ultroser G. Serial subcultivation with this product was possible for at least 10 passages without any change in cell shape and size, saturation density, dome-forming ability, transepithelial resistance, and growth curve. Adhesion of newly plated cells to plastic was somewhat lower than in fetal calf serum but the trypsin-harvesting kinetics were essentially the same. However, the membrane ion transport system was altered: cell sodium influx was greatly diminished, suggesting a deep change in the amiloride-sensitive Na+ channels; sodium efflux was highly enhanced (both active and passive).