中国人群中 CCL2-2518、CXCL10-201、IL8+781 的遗传多态性与肠道病毒 71 感染严重程度的易感性。
Genetic polymorphism of CCL2-2518, CXCL10-201, IL8+781 and susceptibility to severity of Enterovirus-71 infection in a Chinese population.
机构信息
Pediatric Department of the Affiliated Hospital of Qingdao University Medical School, No. 16 Jiangsu Road, Qingdao, 266003, Shandong, People's Republic of China,
出版信息
Inflamm Res. 2014 Jul;63(7):549-56. doi: 10.1007/s00011-014-0724-6. Epub 2014 Mar 9.
OBJECTIVE
The goal of this study was to examine the relationship between CCL2-2518A/G, CXCL10-201A/G, and IL8+781C/T gene polymorphism and severity of Enterovirus 71 (EV71) infection in a Chinese population.
METHODS
A case-control study was conducted to compare the distribution of genotype and genetic frequency of the CCL2-2518A/G, CXCL10-201A/G, and IL8+781C/T gene polymorphisms among EV71-infected patients (n = 186), including mild cases (n = 103), severe cases (n = 83) and healthy control subjects (n = 233) with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and analyzed the relationship between the CCL2-2518A/G, CXCL10-201A/G, and IL8+781C/T gene polymorphism and the susceptibility to EV71 infection.
RESULTS
No significant differences were found in the distribution of genotype CCL2-2518A/G, CXCL10-201A/G, and IL8+781C/T between the healthy control group and EV71-infected patients. However, three SNPs were associated with severity of EV71 infection: the G allele (genotypes AG or GG) in the CCL2-2518A/G (OR 2.34, 95 % CI 1.50-3.65, P < 0.001), the A allele (genotypes AA or AG) in the CXCL10-201A/G (OR 3.60, 95 % CI 1.73-7.47, P < 0.001), and the C allele (genotypes CC or CT) in the IL8+781C/T (OR 2.63, 95 % CI 1.67-4.13, P < 0.001) were more frequent in patients with severe EV71 infection. No significant difference was observed between EV71 encephalitis and severe cases. At the same time, there were significant differences in fever days, WBC, CRP and BG concentration, and CCL2, CXCL10 and IL-8 levels according to the three SNPs among 186 EV71-infected patients, but no significant differences were observed in gender, age, ALT, AST, CK-MB, and CSF evaluations.
CONCLUSION
The G carrier of the CCL2-2518A/G, the A carrier of the CXCL10-201A/G, and the C carrier of the IL8+781C/T were found to be associated with severity of EV71 infection, and could be susceptibility factors in the development of EV71 infection in the Chinese population.
目的
本研究旨在探讨趋化因子（C-C 基元）配体 2（CCL2）-2518A/G、趋化因子（C-X-C 基元）配体 10（CXCL10）-201A/G 和白细胞介素 8（IL8）+781C/T 基因多态性与中国人群中肠道病毒 71（EV71）感染严重程度之间的关系。
方法
采用病例对照研究，比较 EV71 感染患者（n=186）、轻症患者（n=103）、重症患者（n=83）和健康对照组（n=233）中 CCL2-2518A/G、CXCL10-201A/G 和 IL8+781C/T 基因多态性的基因型和遗传频率分布，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 CCL2-2518A/G、CXCL10-201A/G 和 IL8+781C/T 基因多态性与 EV71 感染易感性之间的关系。
结果
健康对照组和 EV71 感染患者的 CCL2-2518A/G、CXCL10-201A/G 和 IL8+781C/T 基因型分布无统计学差异。然而，三个 SNP 与 EV71 感染的严重程度相关：CCL2-2518A/G 中的 G 等位基因（基因型 AG 或 GG）（OR 2.34，95%CI 1.50-3.65，P<0.001），CXCL10-201A/G 中的 A 等位基因（基因型 AA 或 AG）（OR 3.60，95%CI 1.73-7.47，P<0.001），IL8+781C/T 中的 C 等位基因（基因型 CC 或 CT）（OR 2.63，95%CI 1.67-4.13，P<0.001）在重症 EV71 感染患者中更为常见。EV71 脑炎与重症患者之间无显著差异。同时，在 186 例 EV71 感染患者中，根据这三个 SNP 检测到发热天数、白细胞计数（WBC）、C 反应蛋白（CRP）和血糖（BG）浓度以及 CCL2、CXCL10 和 IL-8 水平存在显著差异，但在性别、年龄、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、肌酸激酶同工酶（CK-MB）和脑脊液评估方面无显著差异。
结论
CCL2-2518A/G 的 G 携带者、CXCL10-201A/G 的 A 携带者和 IL8+781C/T 的 C 携带者与 EV71 感染的严重程度有关，可能是中国人群 EV71 感染发展的易感因素。
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