槲皮素促进脂肪源性干细胞的成骨分化。
Osteogenic differentiation of adipose-derived stem cells promoted by quercetin.
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
出版信息
Cell Prolif. 2014 Apr;47(2):124-32. doi: 10.1111/cpr.12097.
OBJECTIVES
The present study aimed to investigate overall effect of quercetin on proliferation and osteogenic differentiation of mouse adipose stem cells (mASCs) in vitro.
MATERIALS AND METHODS
Mouse adipose stem cells were isolated from subcutaneous fat pads and induced into the osteogenic lineage. Effects of quercetin on cell proliferation were assessed using MTT assay. Then they were treated by quercetin for 3, 7 and 11 days in a range of concentrations. Finally, effects of quercetin on osteogenic differentiation of mASCs were analysed by real-time PCR.
RESULTS
Data of MTT assay showed that quercetin did not enhance mASC proliferation in a dose-dependent or time-dependent manner. Results of qPCR indicated that quercetin promoted expressions of Osx, Runx2, BMP-2, Col-1, OPN and OCN at the mRNA level in the presence of osteo-induction medium.
CONCLUSIONS
Our data demonstrated that quercetin was not active in terms of enhancing mASCs proliferation; however, it increased osteogenesis of mASCs by up-regulation of genes including Osx, Runx2, BMP-2, Col-1, OPN and OCN.
目的
本研究旨在探讨槲皮素对体外培养的小鼠脂肪干细胞（mASCs）增殖和成骨分化的整体影响。
材料与方法
从皮下脂肪垫中分离出小鼠脂肪干细胞，并诱导其向成骨谱系分化。采用 MTT 法检测槲皮素对细胞增殖的影响。然后，用不同浓度的槲皮素处理细胞 3、7 和 11 天。最后，通过实时 PCR 分析槲皮素对 mASCs 成骨分化的影响。
结果
MTT 检测结果表明，槲皮素在剂量和时间依赖性方式下均不能促进 mASC 增殖。qPCR 结果表明，在成骨诱导培养基存在的情况下，槲皮素可促进 Osx、Runx2、BMP-2、Col-1、OPN 和 OCN 等基因在 mRNA 水平上的表达。
结论
本研究数据表明，槲皮素在促进 mASCs 增殖方面没有活性；然而，它通过上调 Osx、Runx2、BMP-2、Col-1、OPN 和 OCN 等基因，增加了 mASCs 的成骨作用。
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