Niakani Afsaneh, Farrokhi Farah, Hasanzadeh Shapour
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Department of Basic Veterinary Science, Fculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Iran J Reprod Med. 2013 Oct;11(10):791-800.
Gonadotropin-releasing hormone (GnRH) is a reproductive key hormone. The GnRH analogues are widely used in in vitro fertilization and treatment of sex hormone-depended cancers induced by the materials used in chemotherapeutic agents.
The aim of this study is to evaluate the effects of cyclophosphamide and decapeptyl (analogues of GnRH) on histomorphometry and stereology of testicular tissue as well as gonadotropic and gonadal hormones indices in mice.
For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline. In order to evaluate the tissue changes in testes of the mice, sections were prepared and stained with Hematoxylin-Eosine, Periodic Acid Schief's (PAS) and Oil-Red-O staining techniques.
The cyclophosphamide causes histomorphologic changes in the testicular tissue; whereas such changes by decapeptyl were comparatively mild. The morphometric results revealed significant reduction in diameters of seminiferous tubules (p=0.02), and the stereological results confirmed significant differences in spermatogenesis (SI) as well as rate of tubal differentiation (TDI) indices between experimental and control groups (p=0.001). In addition, the morphometric findings proved that, there are significant decrease (p=0.001) in thicknesses of epithelia and stereologic result revealed reduction in number of cell layers in both decapeptyl and chemotherapy groups, but the decrements of these parameters were significant (p=0.02) in later group. In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
These results demonstrated that, analogue of GnRH i.e., decapeptyl protect morphologic, morphometric, and stereologic alterations of the testes tissue, as well as gonadotropic and gonadal hormonal changes preceding cyclophosphamide treatment in male mice. This article extracted from M.Sc. thesis. (Afsaneh Niakani).
促性腺激素释放激素（GnRH）是一种生殖关键激素。GnRH类似物广泛应用于体外受精以及治疗化疗药物所致的性激素依赖性癌症。
本研究旨在评估环磷酰胺和地加瑞克（GnRH类似物）对小鼠睾丸组织的组织形态计量学和体视学的影响，以及对促性腺激素和性腺激素指标的影响。
本研究中，将24只成年雄性Balb/C品系小鼠分为四组；第一组，给予环磷酰胺（65mg/kg体重），第二组，给予地加瑞克（0.05mg/kg体重），第三组，先给予地加瑞克，在注射环磷酰胺10天后再给予环磷酰胺，对照组给予相同体积的无菌生理盐水。为评估小鼠睾丸组织的变化，制备切片并用苏木精-伊红染色、过碘酸希夫（PAS）染色和油红O染色技术进行染色。
环磷酰胺导致睾丸组织出现组织形态学变化；而地加瑞克所致的此类变化相对较轻。形态计量学结果显示生精小管直径显著减小（p=0.02），体视学结果证实实验组和对照组在精子发生（SI）以及生精小管分化率（TDI）指标上存在显著差异（p=0.001）。此外，形态计量学结果证明，地加瑞克组和化疗组的上皮厚度均显著降低（p=0.001），体视学结果显示细胞层数减少，但后一组这些参数的降低更为显著（p=0.02）。在单独接受环磷酰胺和地加瑞克的组中，促黄体生成素（LH）和睾酮水平显著降低（p=0.03），而在同时接受地加瑞克和环磷酰胺的组中，LH和促卵泡生成素（FSH）水平下降，但睾酮水平升高。
这些结果表明，GnRH类似物即地加瑞克可保护雄性小鼠睾丸组织在环磷酰胺治疗前的形态、形态计量学和体视学改变，以及促性腺激素和性腺激素变化。本文摘自硕士学位论文。（阿芙莎妮·尼亚卡尼）
