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豌豆根防御系统对金属(铜、锌、镉、铅)二元组合的响应。

Response of the pea roots defense systems to the two-element combinations of metals (Cu, Zn, Cd, Pb).

作者信息

Malecka Arleta, Piechalak Aneta, Zielińska Barbara, Kutrowska Agnieszka, Tomaszewska Barbara

机构信息

Department of Biochemistry, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Collegium Biologicum, Poznań, Poland.

出版信息

Acta Biochim Pol. 2014;61(1):23-8. Epub 2014 Mar 22.

Abstract

The presence of the single metals (Cd, Pb, Cu, Zn) induces ROS (reactive oxygen species) production and causes oxidative stress in plants. While applied in two-element combinations, trace metals impact organisms in a more complex way. To assess the resultant effect we treated the pea grown hydroponically with the trace metals in variants: CuPb, CuCd, CuZn, PbCd, ZnPb, ZnCd in concentrations of 25 µM for each metal ion. Abiotic stress inhibited root elongation growth, decreased biomass production, induced changes in root colour and morphology. It changed rate of ROS production, malondialdehyde content, increased activity and altered gene expression of defence enzymes (superoxide dysmutase, catalase, ascorbate peroxidase, glutathione reductase, γ-glutamylcysteine synthetase).

摘要

单一金属(镉、铅、铜、锌)的存在会诱导植物产生活性氧(ROS)并导致氧化应激。当以两种元素组合施用时,微量金属对生物体的影响更为复杂。为了评估其综合效应,我们用不同组合的微量金属对水培豌豆进行处理:铜铅、铜镉、铜锌、铅镉、锌铅、锌镉,每种金属离子的浓度均为25μM。非生物胁迫抑制了根的伸长生长,降低了生物量的产生,诱导了根颜色和形态的变化。它改变了活性氧的产生速率、丙二醛含量,增加了防御酶(超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶、γ-谷氨酰半胱氨酸合成酶)的活性并改变了其基因表达。

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