一种短的HLA - DQβ基因座特异性cDNA探针的克隆：DQw特异性分型
Cloning of a short HLA-DQ beta locus-specific cDNA probe: typing for DQw specificities.
作者信息
Sood S K, McCusker C T, Singal D P
机构信息
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
出版信息
Mol Immunol. 1989 Jan;26(1):101-5. doi: 10.1016/0161-5890(89)90026-6.
A short HLA-DQ beta locus-specific (141 bp) probe was cloned from the full-length pII-beta-1 cDNA. Pst 1-digested genomic DNA from homozygous typing cell lines (HTC) was hybridized with this short DQ beta locus-specific, pDQ beta 141, probe. Restriction fragment length polymorphism (RFLP) patterns generated with this DQ beta locus-specific probe were compared with those obtained with the full-length (627 bp) DQ beta, pII-beta-1, probe. The results demonstrate that the RFLP patterns with the pDQ beta 141 probe were very simple, and no crossreacting DR beta and DX beta bands were observed. DQw1, 2, 3 and 4 specificities could each be identified by a single RFLP.
从全长pII-β-1 cDNA中克隆出一个短的HLA-DQβ基因座特异性（141 bp）探针。用Pst 1消化纯合分型细胞系（HTC）的基因组DNA，并与这个短的DQβ基因座特异性探针pDQβ 141杂交。将用该DQβ基因座特异性探针产生的限制性片段长度多态性（RFLP）模式与用全长（627 bp）DQβ探针pII-β-1获得的模式进行比较。结果表明，pDQβ 141探针的RFLP模式非常简单，未观察到交叉反应的DRβ和DXβ条带。DQw1、2、3和4特异性均可通过单一RFLP鉴定。
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