Spratt Henry G, Levine David, Tillman Larry
Department of Biological and Environmental Sciences and.
Physiother Theory Pract. 2014 Oct;30(7):507-11. doi: 10.3109/09593985.2014.900836. Epub 2014 Mar 28.
A procedure commonly used in physical therapy (PT) clinics is therapeutic ultrasound (US). This equipment and associated gel comes in contact with patient skin, potentially serving as a reservoir for bacteria. In this study, we sampled US heads, gel bottle tips and gel from nine outpatient PT clinics in Southeastern Tennessee. Samples were collected using sterile swabs. At the microbiology laboratory, these swabs were used to inoculate mannitol salt agar and CHROM-MRSA agar (for Staphylococcal species) and tryptic soy broth to determine non-specific bacterial contamination. US heads, gel bottle tips and gel had variable levels of contamination. Tips of gel bottles had the highest contamination, with 52.7% positive for non-specific bacterial contamination and 3.6% positive for methicillin-resistant Staphylococcus aureus (MRSA). Contamination of gel by non-specific bacteria was found in 14.5% of bottles sampled. US heads (35.5% of those sampled) had non-specific bacterial contamination, with no MRSA detected. Disinfecting US heads after initial swabbing resulted in removal of 90.9% of non-specific contamination. Gel storage at temperatures below 40 °C was found to encourage the growth of mesophilic bacteria. This study demonstrates the need for better cleaning and storage protocols for US heads and gel bottles in PT clinics.
物理治疗(PT)诊所常用的一种治疗方法是超声疗法(US)。这种设备及相关凝胶会接触患者皮肤,可能成为细菌滋生地。在本研究中,我们对田纳西州东南部9家门诊PT诊所的超声探头、凝胶瓶瓶口及凝胶进行了采样。样本采集使用无菌拭子。在微生物实验室,这些拭子被用于接种甘露醇盐琼脂和CHROM-MRSA琼脂(用于检测葡萄球菌属)以及胰蛋白胨大豆肉汤,以确定非特异性细菌污染情况。超声探头、凝胶瓶瓶口及凝胶的污染程度各不相同。凝胶瓶瓶口的污染最为严重,非特异性细菌污染阳性率为52.7%,耐甲氧西林金黄色葡萄球菌(MRSA)阳性率为3.6%。在采样的凝胶瓶中,14.5%发现有非特异性细菌污染。超声探头(采样探头的35.5%)存在非特异性细菌污染,未检测到MRSA。初次擦拭后对超声探头进行消毒,可去除90.9%的非特异性污染物。研究发现,凝胶储存在40°C以下的温度会促使嗜温细菌生长。本研究表明,PT诊所的超声探头和凝胶瓶需要更好的清洁和储存方案。