双光子激发提高了厚散射组织中的多焦点结构照明显微镜技术。
Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue.
机构信息
National Institute of Biomedical Imaging and Bioengineering, National Institute of Dental and Craniofacial Research, and National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5254-9. doi: 10.1073/pnas.1314447111. Epub 2014 Mar 24.
Abstract
Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 µm, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.
摘要
多焦点结构光照明显微镜（MSIM）在深度达 50 µm 的样本中提供了两倍于衍射极限的分辨率增强，但在厚样本中散射和离焦光会降低 MSIM 的性能。在这里，我们使用微透镜阵列实现了 MSIM，以实现高效的双光子激发。双光子 MSIM 给出了分辨率加倍的图像，在厚散射样本（如秀丽隐杆线虫胚胎、黑腹果蝇幼虫唾液腺和小鼠肝组织）中具有更好的切片和对比度。
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