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热水解和酶解从肠浒苔中生产还原糖。

Production of reducing sugar from Enteromorpha intestinalis by hydrothermal and enzymatic hydrolysis.

机构信息

Department of Biotechnology, Pukyong National University, Busan 608-737, South Korea.

Department of Biotechnology, Pukyong National University, Busan 608-737, South Korea.

出版信息

Bioresour Technol. 2014 Jun;161:348-53. doi: 10.1016/j.biortech.2014.03.078. Epub 2014 Mar 25.

Abstract

In this work, to evaluate the efficacy of marine macro-algae Enteromorpha intestinalis as a potential bioenergy resource, the effects of reaction conditions (solid-to-liquid ratio, reaction temperature, and reaction time) on sugars produced by a combined process of hydrothermal and enzymatic hydrolysis were investigated. As a result of the hydrothermal hydrolysis, a 7.3g/L (8% yield) total reducing sugar was obtained under conditions including solid-to-liquid ratio of 1:10, reaction temperature of 170°C, and reaction time of 60min. By subsequent (post-hydrothermal) enzymatic hydrolysis of samples treated at 170°C for 30min, a 20.1g/L (22% yield) was achieved.

摘要

在这项工作中,为了评估海洋大型藻类肠浒苔作为潜在生物能源资源的功效,考察了水热和酶解联合过程中反应条件(固液比、反应温度和反应时间)对产生的糖的影响。通过水热水解,在固液比为 1:10、反应温度为 170°C 和反应时间为 60min 的条件下,得到了 7.3g/L(8%产率)的总还原糖。通过对在 170°C 下处理 30min 的样品进行后续(水热后)酶解,得到了 20.1g/L(22%产率)。

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